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题目:: Enrichment of lineage-CD34- cells using a newly developed filter system.
作者:: Nakamura(Y),Ando(K),Muguruma(Y),Sumita(M),Terashima(S),Yoshida(M),Yamashita(Y),Hotta(T),Kato(S)
状态:: 发布时间2000-06-14 , 更新时间 2006-11-15
期刊:: Br J Haematol
摘要:: Recent studies have demonstrated the presence of human lineage- (lin-)CD34- cells that are capable of differentiating into CD34+ cells in xenogeneic transplantation systems. We developed a new filter system that can enrich lin-CD34- cells, a precursor cell population of CD34+ cells. The filter consists of polyethylene terephthalate non-woven fabrics coated with hydrophilic polymer. The frequency of lin-CD34- cells in the cell population after filtration was 7.45 +/- 4.41%, a 16.8 +/- 8.81-fold enrichment compared with 0. 49 +/- 0.31% in the cell population before filtration. The mean recovery of lin-CD34- cells was 48.57 +/- 13.59% (n = 15). Purified lin-CD34- cells, obtained by sorting the filtrated cell population, acquired CD34 expression and colony-forming activity after 7 d of culture. Our results indicate that this filter system is useful for isolating lin-CD34- cells, including precursors of CD34+ cells, and will help further the study of lin-CD34- cells.
语言:: eng
DOI:

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