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PMID: 10790848 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization.

Journal of the American Society for Mass Spectrometry ·Vol. 11 ·No. 5 ·2000-05-00 ·Pages 437-49

Hsu FF, Turk J

Abstract

Alkaline metal adduct ions of sphingomyelin were formed by electrospray ionization in positive ion mode. Under low energy collisionally activated dissociation (CAD), the product ion spectra yield abundant fragment ions representative of both long chain base and fatty acid which permit unequivocal determination of the structure. Tandem spectra obtained by constant neutral loss scanning permit identification of sphingomyelin class and specific long chain base subclass in the mixture. The fragmentation pathways under CAD were proposed, and were further confirmed by source CAD tandem mass spectrometry. The total analysis of sphingomyelin mixtures from bovine brain, bovine erythrocytes, and chicken egg yolk is also presented.

MeSH Terms
Lithium/chemistry Mass Spectrometry Metals/chemistry Molecular Weight Potassium/chemistry Reference Standards Sodium/chemistry Sphingomyelins/chemistry
Chemicals
Metals Sphingomyelins Lithium Sodium Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hsu F F
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA. fhsu@im.wustl.edu
Turk J
References (19)
19 references, click to expand
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Article Info
Journal
Journal of the American Society for Mass Spectrometry
Abbr.
J Am Soc Mass Spectrom
ISSN
1044-0305
DOI
10.1016/S1044-0305(99)00150-6
Email
fhsu@im.wustl.edu
Published
2000-05-00
Pages
437-49
Language
English
Region
United States
NLM ID
9010412
Subset
IM
Grants
NCRR NIH HHS · P41-RR-00954 · United States
NIDDK NIH HHS · P60-DK-20579 · United States
NIDDK NIH HHS · R37-DK-34388 · United States
External Links
PubMed source DOI: 10.1016/S1044-0305(99)00150-6 View journal details
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