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PMID: 10781567 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of the FimB integrase with the fimS invertible DNA element in Escherichia coli in vivo and in vitro.

Journal of bacteriology ·Vol. 182 ·No. 10 ·2000-05-00 ·Pages 2953-9

Burns LS, Smith SG, Dorman CJ

Abstract

The FimB protein is a site-specific recombinase that inverts the fimS genetic switch in Escherichia coli. Based on amino acid sequence analysis alone, FimB has been assigned to the integrase family of tyrosine recombinases. We show that amino acid substitutions at positions R47, H141, R144, and Y176, corresponding to highly conserved members of the catalytic motif of integrase proteins, render FimB incapable of inverting the fimS element in vivo. The arginine substitutions reduced the ability of FimB to bind to fimS in vivo or in vitro, while the substitution R144Q resulted in a protein unable to bind independently to the half sites located at the left end of fimS in phase-on bacteria. These data confirm that FimB is an integrase and suggest that residue R144 has a role in binding to a specific component of the fim switch.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Catalytic Domain Chromosome Inversion DNA, Bacterial/metabolism DNA-Binding Proteins/genetics,metabolism Escherichia coli/genetics Escherichia coli Proteins Integrases/genetics,metabolism Molecular Sequence Data Repetitive Sequences, Nucleic Acid Repressor Proteins
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins Escherichia coli Proteins Repressor Proteins fimB protein, E coli Integrases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Burns L S
Department of Microbiology, Moyne Institute of Preventive Medicine, University of Dublin, Trinity College, Dublin 2, Republic of Ireland.
Smith S G
Dorman C J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.10.2953-2959.2000
Published
2000-05-00
Pages
2953-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC102007
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1128/JB.182.10.2953-2959.2000 PMC Full Text View journal details
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