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PMID: 10779333 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SINE retroposons can be used in vivo as nucleation centers for de novo methylation.

Molecular and cellular biology ·Vol. 20 ·No. 10 ·2000-05-00 ·Pages 3434-41

Arnaud P, Goubely C, Pélissier T, Deragon JM

Abstract

SINEs (short interspersed elements) are an abundant class of transposable elements found in a wide variety of eukaryotes. Using the genomic sequencing technique, we observed that plant S1 SINE retroposons mainly integrate in hypomethylated DNA regions and are targeted by methylases. Methylation can then spread from the SINE into flanking genomic sequences, creating distal epigenetic modifications. This methylation spreading is vectorially directed upstream or downstream of the S1 element, suggesting that it could be facilitated when a potentially good methylatable sequence is single stranded during DNA replication, particularly when located on the lagging strand. Replication of a short methylated DNA region could thus lead to the de novo methylation of upstream or downstream adjacent sequences.

MeSH Terms
5-Methylcytosine Brassica/genetics Cytosine/analogs & derivatives,analysis DNA Methylation DNA Replication DNA, Plant/genetics Genome, Plant Models, Genetic Retroelements Sequence Analysis, DNA Short Interspersed Nucleotide Elements
Chemicals
DNA, Plant Retroelements 5-Methylcytosine Cytosine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Arnaud P
Biomove, UMR6547 CNRS, Université Blaise Pascal Clermont-Ferrand II, 63177 Aubière Cedex, France.
Goubely C
Pélissier T
Deragon J M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.10.3434-3441.2000
Published
2000-05-00
Pages
3434-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC85636
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.20.10.3434-3441.2000 PMC Full Text View journal details
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