Normalization strategies for cDNA microarrays.

Nucleic acids research ·Vol. 28 ·No. 10 ·2000-05-15 ·Pages E47

Schuchhardt J, Beule D, Malik A, Wolski E, Eickhoff H, Lehrach H, Herzel H

Abstract

Multiple Arabidopsis thaliana clones from an experimental series of cDNA microarrays are evaluated in order to identify essential sources of noise in the spotting and hybridization process. Theoretical and experimental strategies for an improved quantitative evaluation of cDNA microarrays are proposed and tested on a series of differently diluted control clones. Several sources of noise are identified from the data. Systematic and stochastic fluctuations in the spotting process are reduced by control spots and statistical techniques. The reliability of slide to slide comparison is critically assessed within the statistical framework of pattern matching and classification.

MeSH Terms

Arabidopsis DNA, Complementary Oligonucleotide Array Sequence Analysis

Chemicals

DNA, Complementary

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Schuchhardt J

Institute for Theoretical Biology, Humboldt-Universität zu Berlin, Invalidenstrasse 43, D-10115 Berlin, Germany. jo@itb.biologie.hu-berlin.de

Beule D

Malik A

Wolski E

Eickhoff H

Lehrach H

Herzel H

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15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

1362-4962

DOI

10.1093/nar/28.10.e47

jo@itb.biologie.hu-berlin.de

Published

2000-05-15

Pages

E47

Language

English

Region

England

NLM ID

0411011

PMCID

PMC105386

Subset

External Links

PubMed source DOI: 10.1093/nar/28.10.e47 PMC Full Text View journal details

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