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PMID: 10760283 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Screening poly(dA/dT)- cDNAs for gene identification.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 8 ·2000-04-11 ·Pages 4162-7

Wang SM, Fears SC, Zhang L, Chen JJ, Rowley JD

Abstract

Many genes expressed in the human genome have not been identified despite intensive efforts. We observed that the presence of long poly(dA/dT) sequences in the 3' end of cDNA templates contributes significantly to this problem, because the hybrids formed randomly between poly(dA) and poly(dT) sequences of unrelated cDNA templates lead to loss of many templates in the normalization/subtraction reactions. The low abundant copies, which account for the majority of the expressed genes, are affected in particular by this phenomenon. We have developed a strategy called screening poly(dA/dT)(-) cDNAs for gene identification to overcome this obstacle. Applying this strategy can significantly enhance the efficiency of genome-wide gene identification and should have an impact on many functional genomic studies in the postgenome era.

MeSH Terms
Base Sequence DNA, Complementary/genetics Expressed Sequence Tags Humans Molecular Sequence Data Poly dA-dT/genetics RNA, Messenger/genetics Subtraction Technique Templates, Genetic
Chemicals
DNA, Complementary RNA, Messenger Poly dA-dT
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wang S M
Section of Hematology and Oncology, University of Chicago Medical Center, 5841 South Maryland Avenue, MC 2115, Chicago, IL 60637-1470, USA. swang1@midway.uchicago.edu
Fears S C
Zhang L
Chen J J
Rowley J D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.97.8.4162
Email
swang1@midway.uchicago.edu
Published
2000-04-11
Pages
4162-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC18183
Subset
IM
Grants
NCI NIH HHS · R01 CA084405 · United States
NCI NIH HHS · CA42557 · United States
NCI NIH HHS · CA78862-01 · United States
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Corrections
CommentIn
PMID: 10760250
External Links
PubMed source DOI: 10.1073/pnas.97.8.4162 PMC Full Text View journal details
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