Fluorescent amplified-fragment length polymorphism genotyping of Salmonella enterica subsp. enterica serovars and comparison with pulsed-field gel electrophoresis typing.

Journal of clinical microbiology ·Vol. 38 ·No. 4 ·2000-04-00 ·Pages 1623-7

Lindstedt BA, Heir E, Vardund T, Kapperud G

Abstract

We have performed the fluorescently labeled amplified-fragment length polymorphism (FAFLP) method on 97 strains of the food-borne pathogen Salmonella enterica subsp. enterica comprising seven different serovars using the restriction enzymes EcoRI and MseI. From the total FAFLP fingerprinted strains, 81 were compared with pulsed-field gel electrophoresis (PFGE) typing of the same strains. The FAFLP method showed a discriminatory power equal to that of PFGE. We report a fast, robust, and high-resolution adaptation of the AFLP assay for fingerprinting S. enterica subsp. enterica serovars with capillary electrophoresis that can be scaled to high throughput on automated analysis instruments.

MeSH Terms

Bacterial Typing Techniques Electrophoresis, Gel, Pulsed-Field Fluorescence Genotype Humans Polymorphism, Restriction Fragment Length Salmonella Food Poisoning/microbiology Salmonella enterica/classification,genetics

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lindstedt B A

Department of Bacteriology, National Institute of Public Health, N-0403 Oslo, Norway. bjorn-arne.lindstedt@flokehelsa.no

Heir E

Vardund T

Kapperud G

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/JCM.38.4.1623-1627.2000

bjorn-arne.lindstedt@flokehelsa.no

Published

2000-04-00

Pages

1623-7

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC86504

Subset

External Links

PubMed source DOI: 10.1128/JCM.38.4.1623-1627.2000 PMC Full Text View journal details

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Fluorescent amplified-fragment length polymorphism genotyping of Salmonella enterica subsp. enterica serovars and comparison with pulsed-field gel electrophoresis typing.

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