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PMID: 10745090 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats.

FEBS letters ·Vol. 470 ·No. 3 ·2000-03-31 ·Pages 325-30

Shiota G, Kunisada T, Oyama K, Udagawa A, Nomi T, Tanaka K, Tsutsumi A, Isono M, Nakamura T, Hamada H, Sakatani T, Sell S, Sato K, Ito H, Kawasaki H

Abstract

To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt-Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased at each time point. Our results suggest that in vivo transfer of the HGF gene into liver accelerates proliferation of hepatic oval cells in the Solt-Farber model in rats.

MeSH Terms

2-Acetylaminofluorene/toxicity Animals Cell Division/drug effects Disease Models, Animal Gene Transfer Techniques Hepatectomy Hepatocyte Growth Factor/genetics,physiology Immunohistochemistry Liver/cytology,drug effects,metabolism,surgery Male Proliferating Cell Nuclear Antigen/analysis Proto-Oncogene Proteins c-kit/genetics Proto-Oncogene Proteins c-met/genetics RNA, Messenger/analysis,genetics Rats Rats, Inbred F344 Stem Cell Factor/genetics Time Factors

Chemicals

Proliferating Cell Nuclear Antigen RNA, Messenger Stem Cell Factor Hepatocyte Growth Factor 2-Acetylaminofluorene Proto-Oncogene Proteins c-kit Proto-Oncogene Proteins c-met

Authors & Affiliations

15 authors, click to expand affiliations / ORCID

Shiota G

Second Department of Internal Medicine, Faculty of Medicine, Tottori University, Yonago, Japan. gshiota@grape.med.tottori-u.ac.jp

Kunisada T

Oyama K

Udagawa A

Nomi T

Tanaka K

Tsutsumi A

Isono M

Nakamura T

Hamada H

Sakatani T

Sell S

Sato K

Ito H

Kawasaki H

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/s0014-5793(00)01337-5

gshiota@grape.med.tottori-u.ac.jp

Published

2000-03-31

Pages

325-30

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/s0014-5793(00)01337-5 View journal details

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In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats.

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