Home LiteratureArticle Details
PMID: 10742267 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Prokaryotic diversity in Zostera noltii-colonized marine sediments.

Applied and environmental microbiology ·Vol. 66 ·No. 4 ·2000-04-00 ·Pages 1715-9

Cifuentes A, Antón J, Benlloch S, Donnelly A, Herbert RA, Rodríguez-Valera F

Abstract

The diversity of microorganisms present in a sediment colonized by the phanerogam Zostera noltii has been analyzed. Microbial DNA was extracted and used for constructing two 16S rDNA clone libraries for Bacteria and Archaea. Bacterial diversity was very high in these samples, since 57 different sequences were found among the 60 clones analyzed. Eight major lineages of the Domain Bacteria were represented in the library. The most frequently retrieved bacterial group (36% of the clones) was delta-Proteobacteria related to sulfate-reducing bacteria. The second most abundant group (27%) was gamma-Proteobacteria, including five clones closely related to S-oxidizing endosymbionts. The archaeal clone library included members of Crenarchaeota and Euryarchaeota, with nine different sequences among the 15 analyzed clones, indicating less diversity when compared to the Bacteria organisms. None of these sequences was closely related to cultured Archaea organisms.

MeSH Terms
Archaea/genetics,growth & development,isolation & purification Bacteria/genetics,growth & development,isolation & purification Cloning, Molecular DNA, Archaeal/analysis,isolation & purification DNA, Bacterial/analysis,isolation & purification DNA, Ribosomal/genetics Gene Library Geologic Sediments/microbiology Molecular Sequence Data Phylogeny Poaceae/growth & development Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Seawater/microbiology Water Microbiology
Chemicals
DNA, Archaeal DNA, Bacterial DNA, Ribosomal RNA, Ribosomal, 16S
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cifuentes A
División de Microbiología, Universidad Miguel Hernández, Campus de San Juan, 03550 San Juan, Alicante, Spain.
Antón J
Benlloch S
Donnelly A
Herbert R A
Rodríguez-Valera F
References (24)
24 references, click to expand
  1. Archaea in coastal marine environments.
    Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5685-9 PMID: 1608980
  2. Phylogenetic analysis of the bacterial communities in marine sediments.
    Appl Environ Microbiol. 1996 Nov;62(11):4049-59 PMID: 8899989
  3. Diversity of free-living and attached bacteria in offshore Western Mediterranean waters as depicted by analysis of genes encoding 16S rRNA.
    Appl Environ Microbiol. 1999 Feb;65(2):514-22 PMID: 9925576
  4. Nitrogen fixation in sediments and the rhizosphere of the seagrassZostera capricorni.
    Microb Ecol. 1991 Dec;22(1):53-64 PMID: 24194325
  5. Phylogenetic and physiological diversity of sulphate-reducing bacteria isolated from a salt marsh sediment.
    Syst Appl Microbiol. 1998 Dec;21(4):557-68 PMID: 9924824
  6. Phylogenetic diversity of Archaea in sediment samples from a coastal salt marsh.
    Appl Environ Microbiol. 1997 Dec;63(12):4729-33 PMID: 9406392
  7. Unexpected population distribution in a microbial mat community: sulfate-reducing bacteria localized to the highly oxic chemocline in contrast to a eukaryotic preference for anoxia.
    Appl Environ Microbiol. 1999 Oct;65(10):4659-65 PMID: 10508103
  8. Sulphate reduction and vertical distribution of sulphate-reducing bacteria quantified by rRNA slot-blot hybridization in a coastal marine sediment.
    Environ Microbiol. 1999 Feb;1(1):65-74 PMID: 11207719
  9. A psychrophilic crenarchaeon inhabits a marine sponge: Cenarchaeum symbiosum gen. nov., sp. nov.
    Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6241-6 PMID: 8692799
  10. The RDP (Ribosomal Database Project).
    Nucleic Acids Res. 1997 Jan 1;25(1):109-11 PMID: 9016515
  11. Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments.
    Appl Environ Microbiol. 1999 Sep;65(9):3976-81 PMID: 10473404
  12. High bacterial diversity in permanently cold marine sediments.
    Appl Environ Microbiol. 1999 Sep;65(9):3982-9 PMID: 10473405
  13. Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
    Microbiol Rev. 1995 Mar;59(1):143-69 PMID: 7535888
  14. Desulfovibrio zosterae sp. nov., a new sulfate reducer isolated from surface-sterilized roots of the seagrass Zostera marina.
    Int J Syst Bacteriol. 1999 Apr;49 Pt 2:859-65 PMID: 10319511
  15. The response of the microbial community of marine sediments to organic carbon input under anaerobic conditions.
    Syst Appl Microbiol. 1999 May;22(2):237-48 PMID: 10390875
  16. Recovery and phylogenetic analysis of archaeal rRNA sequences from continental shelf sediments.
    FEMS Microbiol Lett. 1998 Apr 1;161(1):83-8 PMID: 9561734
  17. Aerobic sulfate reduction in microbial mats.
    Science. 1991 Mar 22;251:1471-3 PMID: 11538266
  18. Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization.
    Appl Environ Microbiol. 1998 Jul 1;64(7):2691-6 PMID: 9647850
  19. Elemental sulfur and thiosulfate disproportionation by Desulfocapsa sulfoexigens sp. nov., a new anaerobic bacterium isolated from marine surface sediment.
    Appl Environ Microbiol. 1998 Jan;64(1):119-25 PMID: 9435068
  20. Bacterial interactions in the rhizosphere of seagrass communities in shallow coastal lagoons.
    J Appl Microbiol. 1998 Dec;85 Suppl 1:151S-160S PMID: 21182704
  21. DNA recovery from soils of diverse composition.
    Appl Environ Microbiol. 1996 Feb;62(2):316-22 PMID: 8593035
  22. Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation.
    Appl Environ Microbiol. 1998 Oct;64(10):3869-77 PMID: 9758812
  23. A phylogenetic tree of 16S rRNA sequences from sulfate-reducing bacteria in a sandy marine sediment.
    Appl Environ Microbiol. 1994 Sep;60(9):3437-9 PMID: 7524446
  24. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.66.4.1715-1719.2000
Published
2000-04-00
Pages
1715-9
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC92048
Subset
IM
Databases
GENBANK
AF218422, AF218423, AF218424, AF218425, AF218426, AF218427, AF218428, AF218429, AF218430, AF223253, AF223254, AF223255, AF223256, AF223257, AF223258, AF223259, AF223260, AF223261, AF223262, AF223263, AF223264, AF223265, AF223266, AF223267, AF223268, AF223269, AF223270, AF223271, AF223272, AF223273
External Links
PubMed source DOI: 10.1128/AEM.66.4.1715-1719.2000 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2