Arrestin: mutagenesis, expression, purification, and functional characterization.

Amino Acid Substitution Animals Arrestin/genetics,isolation & purification,metabolism Blotting, Western/methods Cell-Free System Chromatography, Affinity/methods Chromatography, Ion Exchange/methods Cloning, Molecular/methods Cyclic AMP-Dependent Protein Kinases/metabolism Electrophoresis, Polyacrylamide Gel/methods Escherichia coli Mutagenesis, Site-Directed Phosphorylation Protein Biosynthesis RNA, Messenger/genetics,metabolism Rabbits Recombinant Proteins/biosynthesis,isolation & purification,metabolism Reticulocytes Rhodopsin/metabolism Transcription, Genetic beta-Adrenergic Receptor Kinases

Arrestin RNA, Messenger Recombinant Proteins Rhodopsin Cyclic AMP-Dependent Protein Kinases beta-Adrenergic Receptor Kinases