Assays for activation of recombinant expressed opsins by all-trans-retinals.

Amino Acid Substitution Animals COS Cells Cell Membrane/metabolism GTP-Binding Protein alpha Subunits, Gi-Go/metabolism Guanosine 5'-O-(3-Thiotriphosphate)/metabolism Kinetics Mutagenesis, Site-Directed Recombinant Proteins/chemistry,isolation & purification,metabolism Retinaldehyde/analogs & derivatives,metabolism Rhodopsin/metabolism Rod Opsins/genetics,isolation & purification,metabolism Spectrometry, Fluorescence/methods Transfection

Recombinant Proteins Rod Opsins Guanosine 5'-O-(3-Thiotriphosphate) Rhodopsin GTP-Binding Protein alpha Subunits, Gi-Go Retinaldehyde