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PMID: 10731133 Published · ppublish English Journal Article

A whole-genome assembly of Drosophila.

Science (New York, N.Y.) ·Vol. 287 ·No. 5461 ·2000-03-24 ·Pages 2196-204

Myers EW, Sutton GG, Delcher AL, Dew IM, Fasulo DP, Flanigan MJ, Kravitz SA, Mobarry CM, Reinert KH, Remington KA, Anson EL, Bolanos RA, Chou HH, Jordan CM, Halpern AL, Lonardi S, Beasley EM, Brandon RC, Chen L, Dunn PJ, Lai Z, Liang Y, Nusskern DR, Zhan M, Zhang Q, Zheng X, Rubin GM, Adams MD, Venter JC

Abstract

We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9- megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99. 99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.

MeSH Terms

Algorithms Animals Chromatin/genetics Computational Biology Contig Mapping Drosophila melanogaster/genetics Euchromatin Genes, Insect Genome Heterochromatin/genetics Molecular Sequence Data Physical Chromosome Mapping Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Sequence Tagged Sites

Chemicals

Chromatin Euchromatin Heterochromatin

Authors & Affiliations

29 authors, click to expand affiliations / ORCID

Myers E W

Celera Genomics, Inc., 45 West Gude Drive, Rockville, MD 20850, USA. Gene.Myers@celera.com

Sutton G G

Delcher A L

Dew I M

Fasulo D P

Flanigan M J

Kravitz S A

Mobarry C M

Reinert K H

Remington K A

Anson E L

Bolanos R A

Chou H H

Jordan C M

Halpern A L

Lonardi S

Beasley E M

Brandon R C

Chen L

Dunn P J

Lai Z

Liang Y

Nusskern D R

Zhan M

Zhang Q

Zheng X

Rubin G M

Adams M D

Venter J C

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.287.5461.2196

gene.myers@celera.com

Published

2000-03-24

Pages

2196-204

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

AC012691, AC012692, AC012693, AC012694, AC012695, AC012696, AC012697, AC012698, AC012699, AC012700, AC012701, AC012702, AC012703, AC012704, AC012705, AC012706, AC012707, AC012708, AC012709, AC012710, AC012711, AC012712, AC012713, AC012714, AC012715, AC012716, AC012717, AC012718, AC012719, AC012720

External Links

PubMed source DOI: 10.1126/science.287.5461.2196 View journal details

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A whole-genome assembly of Drosophila.

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