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PMID: 10712533 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Expression of AtPRP3, a proline-rich structural cell wall protein from Arabidopsis, is regulated by cell-type-specific developmental pathways involved in root hair formation.

Plant physiology ·Vol. 122 ·No. 3 ·2000-03-00 ·Pages 705-14

Bernhardt C, Tierney ML

Abstract

The tightly regulated expression patterns of structural cell wall proteins in several plant species indicate that they play a crucial role in determining the extracellular matrix structure for specific cell types. We demonstrate that AtPRP3, a proline-rich cell wall protein in Arabidopsis, is expressed in root-hair-bearing epidermal cells at the root/shoot junction and within the root differentiation zone of light-grown seedlings. Several lines of evidence support a direct relationship between AtPRP3 expression and root hair development. AtPRP3/beta-glucuronidase (GUS) expression increased in roots of transgenic seedlings treated with either 1-aminocyclopropane-1-carboxylic acid (ACC) or alpha-naphthaleneacetic acid (alpha-NAA), compounds known to promote root hair formation. In the presence of 1-alpha-(2-aminoethoxyvinyl)glycine (AVG), an inhibitor of ethylene biosynthesis, AtPRP3/GUS expression was strongly reduced, but could be rescued by co-addition of ACC or alpha-NAA to the growth medium. In addition, AtPRP3/GUS activity was enhanced in ttg and gl2 mutant backgrounds that exhibit ectopic root hairs, but was reduced in rhd6 and 35S-R root-hair-less mutant seedlings. These results indicate that AtPRP3 is regulated by developmental pathways involved in root hair formation, and are consistent with AtPRP3's contributing to cell wall structure in Arabidopsis root hairs.

MeSH Terms
Amino Acids/pharmacology Amino Acids, Cyclic Arabidopsis/drug effects,genetics,growth & development Arabidopsis Proteins Gene Expression Regulation, Developmental/drug effects Gene Expression Regulation, Plant/drug effects Genes, Plant Glucuronidase/genetics Mutation Naphthaleneacetic Acids/pharmacology Plant Growth Regulators/pharmacology Plant Proteins/genetics Plant Roots/growth & development Plants, Genetically Modified Recombinant Fusion Proteins/genetics
Chemicals
Amino Acids Amino Acids, Cyclic Arabidopsis Proteins Naphthaleneacetic Acids Plant Growth Regulators Plant Proteins Recombinant Fusion Proteins proline-rich protein, Arabidopsis 1-naphthaleneacetic acid 1-aminocyclopropane-1-carboxylic acid Glucuronidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bernhardt C
Department of Botany and Agricultural Biochemistry, University of Vermont, Burlington, Vermont 05405, USA.
Tierney M L
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.122.3.705
Published
2000-03-00
Pages
705-14
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC58905
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.122.3.705 PMC Full Text View journal details
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