Home LiteratureArticle Details
PMID: 1069992 Published · ppublish English Journal Article

Histones H3 and H4 interact with the ends of nucleosome DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 12 ·1976-12-00 ·Pages 4400-4

Simpson RT

Abstract

Isolated HeLa cell nucleosomes (core particles) were labeled at the 5'-termini of their DNA with 32P using [gamma-32P]ATP and polynucleotide kne by sequential methylation, depurination, Schiff base formation, and reduction with sodium borhydride. After digestion of the noncrosslinked DNA by DNase I and venom phosphodiesterase, histones were separated by gel electrophoresis and those crosslinked to the 5'-termini were identified by 32P-autoradiography. Histones H3 and H4 occur with equeal frequency as the nearest protein neighbors to the end of the DNA in nucleosomes. Histone arrangements within the core particle compatible with these results are discussed.

MeSH Terms
Base Sequence Chromatin/ultrastructure DNA/metabolism HeLa Cells Histones/metabolism Methods Protein Binding
Chemicals
Chromatin Histones DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Simpson R T
References (35)
35 references, click to expand
  1. An octamer of histones in chromatin and free in solution.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2626-30 PMID: 241077
  2. Histone compostion of chromatin subunits studied by immunosedimentation.
    Biochemistry. 1976 Sep 21;15(19):4305-12 PMID: 822871
  3. Physical studies on the H3/H4 histone tetramer.
    Biochemistry. 1976 Jun 1;15(11):2261-7 PMID: 945069
  4. Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes.
    Biochemistry. 1976 Jul 27;15(15):3307-14 PMID: 952859
  5. Analysis of subunit organization in chicken erythrocyte chromatin.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):505-9 PMID: 1061151
  6. A model for chromatin sub-structure incorporating symmetry considerations of histone oligomers.
    Nucleic Acids Res. 1975 Mar;2(3):405-21 PMID: 1129140
  7. A comparison of the digestion of nuclei and chromatin by staphylococcal nuclease.
    Biochemistry. 1975 Jul;14(13):2915-20 PMID: 1148184
  8. Cleavage of DNA in nuclei and chromatin with staphylococcal nuclease.
    Biochemistry. 1975 Jul;14(13):2921-5 PMID: 1148185
  9. [Covalent binding of proteins with DNA in the chromatin structure].
    Dokl Akad Nauk SSSR. 1975 Apr 11;(5):1222-5 PMID: 1149603
  10. Histone-histone associations within chromatin. Cross-linking studies using tetranitromethane.
    Biochemistry. 1975 Mar 11;14(5):1073-8 PMID: 1168485
  11. An approach to histone nearest neighbours in extended chromatin.
    Nucleic Acids Res. 1975 Oct;2(10):1751-70 PMID: 1187345
  12. Chemical evidence that chromatin DNA exists as 160 base pair beads interspersed with 40 base pair bridges.
    Nucleic Acids Res. 1976 Jan;3(1):117-27 PMID: 1250693
  13. Histone-histone interactions within chromatin. Crosslinking studies using ultraviolet light.
    Biochemistry. 1976 May 4;15(9):2002-7 PMID: 1268203
  14. Subunit structure of chromatin.
    Nature. 1974 Sep 20;251(5472):249-51 PMID: 4422492
  15. Localization of chromatin proteins within DNA grooves by methylation of chromatin with dimethyl sulphate.
    Mol Biol Rep. 1974 Sep;1(7):379-84 PMID: 4472897
  16. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.
    Biochem Biophys Res Commun. 1973 May 15;52(2):504-10 PMID: 4711166
  17. Localization of nucleolar and chromatin residual acidic protein changes during differentiation in Physarum polycephalum.
    Arch Biochem Biophys. 1973 Mar;155(1):144-58 PMID: 4736503
  18. Chromatin structure; oligomers of the histones.
    Science. 1974 May 24;184(4139):865-8 PMID: 4825888
  19. Internal structure of the chromatin subunit.
    Nucleic Acids Res. 1974 Nov;1(11):1573-8 PMID: 10793712
  20. Mapping DNAase l-susceptible sites in nucleosomes labeled at the 5' ends.
    Cell. 1976 Oct;9(2):347-53 PMID: 975247
  21. Kinky helix.
    Nature. 1975 Jun 12;255(5509):530-3 PMID: 1095931
  22. The presence of F3-F2a1 dimers and F1 oligomers in chromatin.
    Biochem Biophys Res Commun. 1975 May 5;64(1):282-8 PMID: 1147923
  23. Urea denaturation of chromatin periodic structure.
    Biochemistry. 1975 Jul 15;14(14):3122-5 PMID: 1148192
  24. Histones H2a, H2b, H3, and H4 form a tetrameric complex in solutions of high salt.
    Cell. 1975 Sep;6(1):85-110 PMID: 1164735
  25. Cleavable cross-links in the analysis of histone-histone associations.
    FEBS Lett. 1975 Oct 15;58(1):353-8 PMID: 1225601
  26. Heterogeneity of chromatin subunits in vitro and location of histone H1.
    Nucleic Acids Res. 1976 Feb;3(2):477-92 PMID: 1257057
  27. Spheroid chromatin units (v bodies).
    Science. 1974 Jan 25;183(4122):330-2 PMID: 4128918
  28. Dissection of chromosome structure with trypsin and nucleases.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4249-53 PMID: 4530301
  29. An approach to the structure of native nucleohistone.
    Biochemistry. 1973 Jan 30;12(3):468-74 PMID: 4734233
  30. A two-subunit histone complex from calf thymus.
    Biochem Biophys Res Commun. 1974 Jul 24;59(2):542-7 PMID: 4859197
  31. Chromatin structure: deduced from a minichromosome.
    Science. 1975 Mar 28;187(4182):1202-3 PMID: 17754289
  32. On the native structure of the histone H3-H4 complex.
    Biochem Biophys Res Commun. 1976 Jan 26;68(2):329-35 PMID: 3176
  33. Structural repeating units in chromatin. I. Evidence for their general occurrence.
    Exp Cell Res. 1976 Jan;97:101-10 PMID: 812708
  34. Histone dimers: a fundamental unit in histone assembly.
    Nucleic Acids Res. 1976 May;3(5):1263-75 PMID: 940769
  35. Preparation and physical characterization of a homogeneous population of monomeric nucleosomes from HeLa cells.
    Nucleic Acids Res. 1976 Sep;3(9):2255-66 PMID: 967693
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.12.4400
Published
1976-12-00
Pages
4400-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431473
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.12.4400 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2