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PMID: 10698955 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing.

The EMBO journal ·Vol. 19 ·No. 5 ·2000-03-01 ·Pages 1148-56

Mazin AV, Zaitseva E, Sung P, Kowalczykowski SC

Abstract

The repair of potentially lethal DNA double-stranded breaks (DSBs) by homologous recombination requires processing of the broken DNA into a resected DNA duplex with a protruding 3'-single-stranded DNA (ssDNA) tail. Accordingly, the canonical models for DSB repair require invasion of an intact homologous DNA template by the 3'-end of the ssDNA, a characteristic that the bacterial pairing protein RecA possesses. Unexpectedly, we find that for the eukaryotic homolog, Rad51 protein, the 5'-end of ssDNA is more invasive than the 3'-end. This pairing bias is unaffected by Rad52, Rad54 or Rad55-57 proteins. However, further investigation reveals that, in contrast to RecA protein, the preferred DNA substrate for Rad51 protein is not ssDNA but rather dsDNA with ssDNA tails. This important distinction permits the Rad51 proteins to promote DNA strand invasion using either 3'- or 5'-ends with similar efficiency.

MeSH Terms
Base Pairing DNA/genetics DNA Repair DNA-Binding Proteins/genetics Escherichia coli Humans Rad51 Recombinase Recombination, Genetic Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins
Chemicals
DNA-Binding Proteins Saccharomyces cerevisiae Proteins DNA RAD51 protein, S cerevisiae RAD51 protein, human Rad51 Recombinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mazin A V
Division of Biological Sciences, Sections of Microbiology, University of California, Davis, CA 95616-8665, USA.
Zaitseva E
Sung P
Kowalczykowski S C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.5.1148
Published
2000-03-01
Pages
1148-56
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305653
Subset
IM
Grants
NIGMS NIH HHS · R01 GM062653 · United States
NIAID NIH HHS · AI-18987 · United States
External Links
PubMed source DOI: 10.1093/emboj/19.5.1148 PMC Full Text View journal details
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