Superstructure of linear duplex DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 11 ·1976-11-00 ·Pages 4125-9

Vollenweider HJ, Koller T, Parello J, Sogo JM

Abstract

The superstructure of a covalently closed circular DNA (of bacteriophage PM 2) was compared by electron microscopy with that of a linear duplex DNA (of bacteriophage T7) when ionic strength and benzyldimethylalkylammonium chloride concentration were varied. In parallel studies the sedimentation behavior of these DNAs was studied by analytical ultracentrifugation, but for technical reasons these had to be without benzyldimethylalkylammonium chloride. By combining the information from the two methods one has to conclude that with increasing ionic strength the linear duplex T7 DNA spontaneously forms a structure similar to that of the superhelical structure of closed circular PM 2 DNA. The superstructure is destroyed under premelting conditions and in the presence of an excess of ethidium bromide.

MeSH Terms

Coliphages DNA, Viral Microscopy, Electron Nucleic Acid Conformation Osmolar Concentration Ultracentrifugation

Chemicals

DNA, Viral

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Vollenweider H J

Koller T

Parello J

Sogo J M

References (25)

25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.11.4125

Published

1976-11-00

Pages

4125-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431353

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.11.4125 PMC Full Text View journal details

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