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PMID: 10684902 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Long-lasting depolarizations in mitral cells of the rat olfactory bulb.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 20 ·No. 5 ·2000-03-01 ·Pages 2011-21

Carlson GC, Shipley MT, Keller A

Abstract

We investigated the mechanisms of long-lasting depolarizing potentials (LLDs) generated in mitral cells with whole-cell patch recordings in the rat olfactory bulb slice. LLDs occur spontaneously and are evoked by either orthodromic stimulation of the olfactory nerve or antidromic stimulation of mitral and tufted (M/T) cells. LLDs are followed by a long refractory period, limiting LLD generation to approximately 1 Hz. LLD production does not appear to involve either intrinsic voltage-activated or metabotropic mechanisms. The initiation of LLDs requires activation of non-NMDA but not NMDA receptors. Dual recordings from the apical dendrites and somata of mitral cells show that LLDs are generated in the distal portion of the apical dendrite, most likely in the glomerulus. The rising phase of LLDs shows characteristics of polyneuronal input, including a high variability and sensitivity to charge screening. Paired recordings from adjacent mitral cells suggest that LLDs occur synchronously only in cells whose apical dendrites ramify in the same glomerulus. These findings suggest that LLDs involve recurrent, intraglomerular dendrodendritic interactions among M/T cells.

MeSH Terms
2-Amino-5-phosphonovalerate/pharmacology 6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology Action Potentials/drug effects,physiology Animals Bicuculline/pharmacology Dendrites/chemistry,physiology Excitatory Amino Acid Agonists/pharmacology Excitatory Amino Acid Antagonists/pharmacology Female GABA Antagonists/pharmacology In Vitro Techniques Kainic Acid/pharmacology Male N-Methylaspartate/pharmacology Olfactory Bulb/cytology,physiology Olfactory Receptor Neurons/physiology,ultrastructure Patch-Clamp Techniques Rats Rats, Wistar Receptors, AMPA/physiology Receptors, Kainic Acid/physiology Receptors, N-Methyl-D-Aspartate/physiology gamma-Aminobutyric Acid/physiology
Chemicals
Excitatory Amino Acid Agonists Excitatory Amino Acid Antagonists GABA Antagonists Receptors, AMPA Receptors, Kainic Acid Receptors, N-Methyl-D-Aspartate gamma-Aminobutyric Acid N-Methylaspartate 6-Cyano-7-nitroquinoxaline-2,3-dione 2-Amino-5-phosphonovalerate Kainic Acid Bicuculline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Carlson G C
Department of Anatomy, Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
Shipley M T
Keller A
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Published
2000-03-01
Pages
2011-21
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6772924
Subset
IM
Grants
NIDCD NIH HHS · F32 DC000347 · United States
NINDS NIH HHS · NS-35360 · United States
NINDS NIH HHS · R01 NS036940 · United States
NIDCD NIH HHS · P01 DC000347 · United States
NINDS NIH HHS · R01 NS035360 · United States
NINDS NIH HHS · NS 36940 · United States
NINDS NIH HHS · NS-31078 · United States
NINDS NIH HHS · R01 NS031078 · United States
External Links
PubMed source PMC Full Text View journal details
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