A comparison of the substrate and electron-donor specificities of the methane mono-oxygenases from three strains of methane-oxidizing bacteria.

The Biochemical journal ·Vol. 177 ·No. 1 ·1979-01-01 ·Pages 361-4

Stirling DI, Colby J, Dalton H

Abstract

1. Methane mono-oxygenase from Methylosinus trichosporium has the same broad substrate specificity as the analogous enzyme from Methylococcus capsulatus (Bath); the enzyme from Methylomonas methanica is more specific. 2. Contrary to previous reports, NAD(P)H and not ascorbate is the required electron donor for the enzyme from Methylosinus trichosporium. 3. It is concluded that these three bacteria contain similar methane mono-oxygenases.

MeSH Terms

Methane Methylococcaceae/enzymology Oxidation-Reduction Oxygenases/metabolism Substrate Specificity

Chemicals

Oxygenases Methane

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Stirling D I

Colby J

Dalton H

References (8)

8 references, click to expand

Quantitative method for the gas chromatographic analysis of short-chain monocarboxylic and dicarboxylic acids in fermentation media.
Appl Microbiol. 1975 Mar;29(3):374-81 PMID: 1167776
Purification and properties of the methane mono-oxygenase enzyme system from Methylosinus trichosporium OB3b.
Biochem J. 1977 Feb 1;161(2):333-44 PMID: 15544
An improved assay for bacterial methane mono-oxygenase: some properties of the enzyme from Methylomonas methanica.
Biochem J. 1975 Nov;151(2):459-62 PMID: 3171
Effect of metal-binding and other compounds on methane oxidation by two strains of Methylococcus capsulatus.
Arch Microbiol. 1977 Jul 26;114(1):71-6 PMID: 410382
The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds.
Biochem J. 1977 Aug 1;165(2):395-402 PMID: 411486
Resolution of the methane mono-oxygenase of Methylococcus capsulatus (Bath) into three components. Purification and properties of component C, a flavoprotein.
Biochem J. 1978 May 1;171(2):461-8 PMID: 418777
Oxidation of carbon monoxide and methane by Pseudomonas methanica.
J Gen Microbiol. 1975 Nov;91(1):79-91 PMID: 467
Some properties of a soluble methane mono-oxygenase from Methylococcus capsulatus strain Bath.
Biochem J. 1976 Aug 1;157(2):495-7 PMID: 962879

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1770361

Published

1979-01-01

Pages

361-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1186376

Subset

External Links

PubMed source DOI: 10.1042/bj1770361 PMC Full Text View journal details

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A comparison of the substrate and electron-donor specificities of the methane mono-oxygenases from three strains of methane-oxidizing bacteria.

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