Home LiteratureArticle Details
PMID: 10679015 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Conserved composition of mammalian box H/ACA and box C/D small nucleolar ribonucleoprotein particles and their interaction with the common factor Nopp140.

Molecular biology of the cell ·Vol. 11 ·No. 2 ·2000-02-00 ·Pages 567-77

Yang Y, Isaac C, Wang C, Dragon F, Pogacic V, Meier UT

Abstract

Small nucleolar ribonucleoprotein particles (snoRNPs) mainly catalyze the modification of rRNA. The two major classes of snoRNPs, box H/ACA and box C/D, function in the pseudouridylation and 2'-O-methylation, respectively, of specific nucleotides. The emerging view based on studies in yeast is that each class of snoRNPs is composed of a unique set of proteins. Here we present a characterization of mammalian snoRNPs. We show that the previously characterized NAP57 is specific for box H/ACA snoRNPs, whereas the newly identified NAP65, the rat homologue of yeast Nop5/58p, is a component of the box C/D class. Using coimmunoprecipitation experiments, we show that the nucleolar and coiled-body protein Nopp140 interacts with both classes of snoRNPs. This interaction is corroborated in vivo by the exclusive depletion of snoRNP proteins from nucleoli in cells transfected with a dominant negative Nopp140 construct. Interestingly, RNA polymerase I transcription is arrested in nucleoli depleted of snoRNPs, raising the possibility of a feedback mechanism between rRNA modification and transcription. Moreover, the Nopp140-snoRNP interaction appears to be conserved in yeast, because depletion of Srp40p, the yeast Nopp140 homologue, in a conditional lethal strain induces the loss of box H/ACA small nucleolar RNAs. We propose that Nopp140 functions as a chaperone of snoRNPs in yeast and vertebrate cells.

MeSH Terms
Amino Acid Sequence Animals COS Cells Cell Nucleolus/chemistry,enzymology,metabolism Conserved Sequence/genetics Epistasis, Genetic Genetic Complementation Test Hydro-Lyases Microtubule-Associated Proteins/genetics,metabolism Molecular Chaperones/genetics,metabolism Molecular Sequence Data Molecular Weight Mutation/genetics Nuclear Proteins/chemistry,deficiency,genetics,metabolism Phosphoproteins/deficiency,genetics,metabolism Protein Binding RNA Polymerase I/metabolism RNA-Binding Proteins/genetics,metabolism Rats Ribonucleoproteins/chemistry,metabolism Ribonucleoproteins, Small Nuclear Ribonucleoproteins, Small Nucleolar/chemistry,deficiency,genetics,metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Serine-Arginine Splicing Factors Transcription, Genetic/genetics,physiology
Chemicals
Microtubule-Associated Proteins Molecular Chaperones NAP57 NOLC1 protein, human NOP58 protein, S cerevisiae Nolc1 protein, rat Nop58 protein, rat Nuclear Proteins Phosphoproteins RNA-Binding Proteins Ribonucleoproteins Ribonucleoproteins, Small Nuclear Ribonucleoproteins, Small Nucleolar Saccharomyces cerevisiae Proteins Serine-Arginine Splicing Factors RNA Polymerase I Hydro-Lyases CBF5 protein, S cerevisiae
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yang Y
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Isaac C
Wang C
Dragon F
Pogacic V
Meier U T
References (50)
50 references, click to expand
  1. Nopp140 functions as a molecular link between the nucleolus and the coiled bodies.
    J Cell Biol. 1998 Jul 27;142(2):319-29 PMID: 9679133
  2. Nop5p is a small nucleolar ribonucleoprotein component required for pre-18 S rRNA processing in yeast.
    J Biol Chem. 1998 Jun 26;273(26):16453-63 PMID: 9632712
  3. Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure.
    RNA. 1998 Dec;4(12):1549-68 PMID: 9848653
  4. Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability.
    RNA. 1999 Mar;5(3):455-67 PMID: 10094313
  5. The nucleolar antigen Nop52, the human homologue of the yeast ribosomal RNA processing RRP1, is recruited at late stages of nucleologenesis.
    J Cell Sci. 1999 Jun;112 ( Pt 12):1889-900 PMID: 10341208
  6. Localization of DNA-dependent RNA polymerase activities in fixed human fibroblasts by autoradiography.
    Exp Cell Res. 1973 Jan;76(1):223-8 PMID: 4682648
  7. Gel protein stains: silver stain.
    Methods Enzymol. 1984;104:441-7 PMID: 6201698
  8. The structure of an antigenic determinant in a protein.
    Cell. 1984 Jul;37(3):767-78 PMID: 6204768
  9. Purification and partial characterization of a nucleolar scleroderma antigen (Mr = 34,000; pI, 8.5) rich in NG,NG-dimethylarginine.
    J Biol Chem. 1985 Nov 15;260(26):14304-10 PMID: 2414294
  10. Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA.
    Mol Cell Biol. 1987 Aug;7(8):2899-913 PMID: 2959855
  11. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein.
    J Cell Biol. 1988 Jul;107(1):17-31 PMID: 3292539
  12. U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
    EMBO J. 1989 Oct;8(10):3113-9 PMID: 2531075
  13. A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae.
    Nucleic Acids Res. 1990 May 25;18(10):3091-2 PMID: 2190191
  14. The numerous modified nucleotides in eukaryotic ribosomal RNA.
    Prog Nucleic Acid Res Mol Biol. 1990;39:241-303 PMID: 2247610
  15. Mouse U14 snRNA is encoded in an intron of the mouse cognate hsc70 heat shock gene.
    Nucleic Acids Res. 1990 Nov 25;18(22):6565-71 PMID: 2251119
  16. A nuclear localization signal binding protein in the nucleolus.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2235-45 PMID: 2177472
  17. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant yeast.
    Methods Enzymol. 1991;194:302-18 PMID: 2005795
  18. Evolutionary conservation of the human nucleolar protein fibrillarin and its functional expression in yeast.
    J Cell Biol. 1991 May;113(4):715-29 PMID: 2026646
  19. GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast.
    EMBO J. 1992 Feb;11(2):673-82 PMID: 1531632
  20. Nopp140 shuttles on tracks between nucleolus and cytoplasm.
    Cell. 1992 Jul 10;70(1):127-38 PMID: 1623516
  21. Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus.
    J Cell Biol. 1993 Jul;122(2):283-93 PMID: 8320255
  22. An essential yeast protein, CBF5p, binds in vitro to centromeres and microtubules.
    Mol Cell Biol. 1993 Aug;13(8):4884-93 PMID: 8336724
  23. Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae.
    Genes Dev. 1994 May 1;8(9):1087-105 PMID: 7926789
  24. Clustering of pseudouridine residues around the peptidyltransferase center of yeast cytoplasmic and mitochondrial ribosomes.
    Biochemistry. 1994 Nov 15;33(45):13475-83 PMID: 7947756
  25. NAP57, a mammalian nucleolar protein with a putative homolog in yeast and bacteria.
    J Cell Biol. 1994 Dec;127(6 Pt 1):1505-14 PMID: 7798307
  26. Regulation of casein kinase II by growth factors: a reevaluation.
    Cell Mol Biol Res. 1994;40(5-6):373-81 PMID: 7735311
  27. Isolation and characterization of the small nucleolar ribonucleoprotein particle snR30 from Saccharomyces cerevisiae.
    J Biol Chem. 1995 May 12;270(19):11549-54 PMID: 7744794
  28. Comparative expressed-sequence-tag analysis of differential gene expression profiles in PC-12 cells before and after nerve growth factor treatment.
    Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8303-7 PMID: 7667285
  29. The small nucleolar RNAs.
    Annu Rev Biochem. 1995;64:897-934 PMID: 7574504
  30. Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli.
    RNA. 1995 Mar;1(1):102-12 PMID: 7489483
  31. A mammalian gene with introns instead of exons generating stable RNA products.
    Nature. 1996 Feb 1;379(6564):464-6 PMID: 8559254
  32. Pseudouridine synthases: four families of enzymes containing a putative uridine-binding motif also conserved in dUTPases and dCTP deaminases.
    Nucleic Acids Res. 1996 Jun 15;24(12):2411-5 PMID: 8710514
  33. Comparison of the rat nucleolar protein nopp140 with its yeast homolog SRP40. Differential phosphorylation in vertebrates and yeast.
    J Biol Chem. 1996 Aug 9;271(32):19376-84 PMID: 8702624
  34. BCM Search Launcher--an integrated interface to molecular biology data base search and analysis services available on the World Wide Web.
    Genome Res. 1996 May;6(5):454-62 PMID: 8743995
  35. The SR protein family: pleiotropic functions in pre-mRNA splicing.
    Trends Biochem Sci. 1996 Aug;21(8):296-301 PMID: 8772383
  36. The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions.
    Cell. 1996 Sep 6;86(5):823-34 PMID: 8797828
  37. Identification and characterization of a nucleolar phosphoprotein, Nopp140, as a transcription factor.
    Mol Cell Biol. 1997 Jan;17(1):230-9 PMID: 8972203
  38. Specific interaction between casein kinase 2 and the nucleolar protein Nopp140.
    J Biol Chem. 1997 Feb 7;272(6):3773-9 PMID: 9013635
  39. Intracellular localization and unique conserved sequences of three small nucleolar RNAs.
    Nucleic Acids Res. 1997 Apr 15;25(8):1591-6 PMID: 9092667
  40. The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation.
    Genes Dev. 1997 Apr 1;11(7):941-56 PMID: 9106664
  41. Function and synthesis of small nucleolar RNAs.
    Curr Opin Cell Biol. 1997 Jun;9(3):337-42 PMID: 9159079
  42. Sno storm in the nucleolus: new roles for myriad small RNPs.
    Cell. 1997 May 30;89(5):669-72 PMID: 9182752
  43. The yeast nucleolar protein Cbf5p is involved in rRNA biosynthesis and interacts genetically with the RNA polymerase I transcription factor RRN3.
    Mol Cell Biol. 1997 Oct;17(10):6175-83 PMID: 9315678
  44. The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis.
    Cell. 1997 Sep 19;90(6):1023-9 PMID: 9323130
  45. Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis.
    Mol Cell Biol. 1997 Dec;17(12):7088-98 PMID: 9372940
  46. In vivo identification of nuclear factors interacting with the conserved elements of box C/D small nucleolar RNAs.
    Mol Cell Biol. 1998 Feb;18(2):1023-8 PMID: 9447999
  47. The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase.
    Genes Dev. 1998 Feb 15;12(4):527-37 PMID: 9472021
  48. In vitro assembly of the mouse U14 snoRNP core complex and identification of a 65-kDa box C/D-binding protein.
    RNA. 1998 May;4(5):582-93 PMID: 9582099
  49. X-linked dyskeratosis congenita is caused by mutations in a highly conserved gene with putative nucleolar functions.
    Nat Genet. 1998 May;19(1):32-8 PMID: 9590285
  50. Nhp2p and Nop10p are essential for the function of H/ACA snoRNPs.
    EMBO J. 1998 Dec 1;17(23):7078-90 PMID: 9843512
Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.11.2.567
Published
2000-02-00
Pages
567-77
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC14794
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.11.2.567 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2