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PMID: 10655416 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparative evaluation of two branched-DNA human immunodeficiency virus type 1 RNA quantification assays with lower detection limits of 50 and 500 copies per milliliter.

Journal of clinical microbiology ·Vol. 38 ·No. 2 ·2000-02-00 ·Pages 914-7

Manegold C, Krempe C, Jablonowski H, Kajala L, Dietrich M, Adams O

Abstract

We have comparatively evaluated Quantiplex version 3.0 and version 2. 0 on 133 plasma samples and a repetitive dilution series. Version 3. 0 yielded higher human immunodeficiency virus RNA values, and the ratio of version 3.0 results to version 2.0 results decreased from 3. 47 below 1,000 copies/ml to 1.97 above 50,000 copies/ml [linear regression, log (version 3.0) = 0.915 + 0.871 x log (version 2.0); r(2) = 0.952].

MeSH Terms
DNA Probes Evaluation Studies as Topic HIV Infections/virology HIV-1/genetics,physiology Humans Nucleic Acid Hybridization RNA, Viral/blood,genetics Reagent Kits, Diagnostic Viral Load
Chemicals
DNA Probes RNA, Viral Reagent Kits, Diagnostic
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Manegold C
Klinik für Gastroenterologie, Hepatologie und Infektiologie, Heinrich-Heine-Universität, Düsseldorf, Germany. manegold@bni.uni-hamburg.de
Krempe C
Jablonowski H
Kajala L
Dietrich M
Adams O
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.38.2.914-917.2000
Email
manegold@bni.uni-hamburg.de
Published
2000-02-00
Pages
914-7
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC86246
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.38.2.914-917.2000 PMC Full Text View journal details
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