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PMID: 1064874 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stimulation of fetal hemoglobin synthesis in bone marrow cultures from adult individuals.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 6 ·1976-06-00 ·Pages 2033-7

Papayannopoulou TH, Brice M, Stamatoyannopoulos G

Abstract

The regulation of fetal hemoglobin in adult erythroid cells was investigated with bone marrow cultures. Fetal hemoglobin (Hb F) was identified in individual erythroid colonies with fluorescent antibodies against Hb F and synthesis of gamma chains was determined with analyses of radioactive globins. The appearance of fetal hemoglobin in erythroid colonies was clonal. All the cells of the Hb F synthesizing colonies contained fetal hemoglobin. The frequency of erythroid colonies showing Hb F was higher than expected compared to the frequency of Hb F containing cells in the blood. Production of Hb F in culture, as shown by analysis of the radioactive globins, was 5 to 14 times higher than baseline Hb F synthesis. These results suggest that the ability for gamma chain synthesis in erythroid cells is determined at or above the level of the precursor cell from which the erythroid colonies, in vitro, derive (probably an erythropoietin responsive stem cell), and that stimulation of fetal hemoglobin synthesis in adult erythroid cells is possible.

MeSH Terms
Aging Bone Marrow/metabolism Bone Marrow Cells Cells, Cultured Clone Cells/metabolism Fetal Hemoglobin/biosynthesis Humans
Chemicals
Fetal Hemoglobin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Papayannopoulou T H
Brice M
Stamatoyannopoulos G
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.6.2033
Published
1976-06-00
Pages
2033-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430442
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.6.2033 PMC Full Text View journal details
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