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PMID: 10639463 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of immunodominant antigens from Helicobacter pylori and evaluation of their reactivities with sera from patients with different gastroduodenal pathologies.

Infection and immunity ·Vol. 68 ·No. 2 ·2000-02-00 ·Pages 915-20

Kimmel B, Bosserhoff A, Frank R, Gross R, Goebel W, Beier D

Abstract

Colonization of the gastric mucosa by Helicobacter pylori is the major cause of gastroduodenal pathologies in humans. Studying the outcome of the humoral immune response directed against this gastric pathogen may contribute substantially to vaccine development and to the improvement of diagnostic techniques based on serology. By using two-dimensional gel electrophoresis, 29 proteins from H. pylori G27 were identified which strongly react with sera derived from H. pylori-infected patients suffering from different gastroduodenal pathologies. These antigens were characterized by mass spectrometry and proved to correspond to products of open reading frames predicted by the H. pylori genome sequence. The comparison of the antigenic patterns recognized by these sera revealed no association of specific H. pylori antigens with antibodies in patients with particular gastroduodenal pathologies.

MeSH Terms
Antibodies, Bacterial/immunology Antigens, Bacterial/analysis,immunology Bacterial Proteins/analysis,immunology Gastritis/immunology Helicobacter pylori/immunology Humans Immunoblotting Lymphoma, B-Cell, Marginal Zone/immunology Peptic Ulcer/immunology Stomach Neoplasms/immunology
Chemicals
Antibodies, Bacterial Antigens, Bacterial Bacterial Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kimmel B
Theodor-Boveri-Institut für Biowissenschaften, Lehrstuhl für Mikrobiologie, Universität Würzburg, D-97074 Würzburg, Germany.
Bosserhoff A
Frank R
Gross R
Goebel W
Beier D
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.68.2.915-920.2000
Published
2000-02-00
Pages
915-20
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC97222
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.68.2.915-920.2000 PMC Full Text View journal details
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