Fluorometric assay of vasopressin and oxytocin: a general approach to the assay of peptides in tissues.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 4 ·1976-04-00 ·Pages 1314-8

Gruber KA, Stein S, Brink L, Radhakrishnan A, Udenfriend S

Abstract

A fluorometric method for the quantitative assay of vasopressin and oxytocin in individual rat pituitaries has been developed. Acid extracts of pituitaries are freed of amino acids and polyamines by passage over a copper-Sephadex column, and the peptides fraction is then labeled by reaction with fluorescamine. The resulting peptide fluorophors are separated by chromatography on a reverse-phase bonded column. Specificity of the procedure was ascertained by several criteria, including bioassay and amino-acid analysis of the eluted peptide fluorophors. The procedure serves as a model system for the assay of tissue peptides in the picomole range.

MeSH Terms

Amino Acids/analysis Animals Chromatography/methods Fluorescent Dyes Male Oxytocin/analysis Peptides/isolation & purification Pituitary Gland/analysis Rats Vasopressins/analysis

Chemicals

Amino Acids Fluorescent Dyes Peptides Vasopressins Oxytocin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Gruber K A

Stein S

Brink L

Radhakrishnan A

Udenfriend S

References (9)

9 references, click to expand

SEPARATION OF AMINO ACIDS, AS COPPER CHELATES, FROM AMINO ACID, PROTEIN AND PEPTIDE MIXTURES.
Anal Biochem. 1965 Aug;12:290-5 PMID: 14340020
Amino acid analysis with fluorescamine of stained protein bands from polyacrylamide gels.
Anal Biochem. 1974 Jul;60(1):272-7 PMID: 4136518
Fluorescent labeling of proteins. A new methodology.
Biochem Biophys Res Commun. 1973 Oct 1;54(3):899-906 PMID: 4148130
Removal of a Fluoram 1-positive impurity from hydrochloric acid.
Anal Biochem. 1974 Sep;61(1):302-4 PMID: 4415552
A simplified protein assay with fluorescamine in samples containing interfering material.
Anal Biochem. 1974 Apr;58(2):559-62 PMID: 4857117
Amino acid analysis with fluorescamine at the picomole level.
Arch Biochem Biophys. 1973 Mar;155(1):202-12 PMID: 4736504
Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.
Science. 1972 Nov 24;178(4063):871-2 PMID: 5085985
Supraoptic neurosecretory neurons of the guinea pig in organ culture. Biosynthesis of vasopressin and neurophysin.
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2782-6 PMID: 5288257
Automatic Monitoring of primary amines in preparative column effluents with fluorescamine.
Anal Biochem. 1975 Aug;67(2):438-45 PMID: 1172383

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.4.1314

Published

1976-04-00

Pages

1314-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430258

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.4.1314 PMC Full Text View journal details

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