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PMID: 10629050 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bid induces the oligomerization and insertion of Bax into the outer mitochondrial membrane.

Molecular and cellular biology ·Vol. 20 ·No. 3 ·2000-02-00 ·Pages 929-35

Eskes R, Desagher S, Antonsson B, Martinou JC

Abstract

In many types of apoptosis, the proapoptotic protein Bax undergoes a change in conformation at the level of the mitochondria. This event always precedes the release of mitochondrial cytochrome c, which, in the cytosol, activates caspases through binding to Apaf-1. The mechanisms by which Bax triggers cytochrome c release are unknown. Here we show that following binding to the BH3-domain-only proapoptotic protein Bid, Bax oligomerizes and then integrates in the outer mitochondrial membrane, where it triggers cytochrome c release. Bax mitochondrial membrane insertion triggered by Bid may represent a key step in pathways leading to apoptosis.

MeSH Terms
Apoptosis BH3 Interacting Domain Death Agonist Protein Carrier Proteins/metabolism Cytochrome c Group/metabolism Digitonin/pharmacology Genes, bcl-2 HeLa Cells Humans Intracellular Membranes/drug effects,metabolism Mitochondria/drug effects,metabolism Models, Biological Protein Structure, Quaternary Proto-Oncogene Proteins/chemistry,metabolism Proto-Oncogene Proteins c-bcl-2/genetics,metabolism Signal Transduction Staurosporine/pharmacology bcl-2-Associated X Protein
Chemicals
BAX protein, human BH3 Interacting Domain Death Agonist Protein BID protein, human Carrier Proteins Cytochrome c Group Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 bcl-2-Associated X Protein Staurosporine Digitonin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Eskes R
Serono Pharmaceutical Research Institute, Ares Serono International S.A., CH-1228 Plan-les Ouates, Geneva, Switzerland.
Desagher S
Antonsson B
Martinou J C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.3.929-935.2000
Published
2000-02-00
Pages
929-35
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC85210
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.20.3.929-935.2000 PMC Full Text View journal details
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