Nuclear magnetic resonance studies of the helix-coil transition of poly (dA-dT) in aqueous solution.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 3 ·1976-03-00 ·Pages 674-8

Patel DJ, Canuel L

Abstract

The well-resolved base and sugar proton resonances in the high resolution proton nuclear magnetic resonance (NMR) spectra of poly(dA-dT) can be monitored during the helix-coil transition. The observable resonances shift upfield on helix formation and the temperature-dependent chemical shifts exhibit a melting temperature t 1/2 = 69.9 +/- 0.3 degrees for 18.8 mM (with respect to phosphorus) poly(dA-dT) in 0.5 M Tris, 0.1 M cacodylate, D2O, pH 7.05. The observable protons are in fast exchange throughout the poly (dA-dT) helix-coil transition. The adenine H2 resonance that shifts upfield by about 1 ppm on helix formation exhibits uncertainty broadening in the fast exchange region...

MeSH Terms

Magnetic Resonance Spectroscopy Nucleic Acid Conformation Phosphorus Poly A Polydeoxyribonucleotides Protons Solutions

Chemicals

Polydeoxyribonucleotides Protons Solutions Poly A Phosphorus

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Patel D J

Canuel L

References (26)

26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.3.674

Published

1976-03-00

Pages

674-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC335980

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.3.674 PMC Full Text View journal details

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Nuclear magnetic resonance studies of the helix-coil transition of poly (dA-dT) in aqueous solution.

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