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PMID: 10619023 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator.

Molecular cell ·Vol. 4 ·No. 5 ·1999-11-00 ·Pages 757-69

Kanuka H, Sawamoto K, Inohara N, Matsuno K, Okano H, Miura M

Abstract

We identified a Drosophila Apaf-1/CED-4 homolog gene, dapaf-1. Alternative splicing results in two dapaf-1 mRNA species, which encode distinct forms of caspase activator, Dapaf-1L (Apaf-1 type) and Dapaf-1S (CED-4 type). Distinct caspases were activated by these Dapaf-1 isoforms. Loss of Dapaf-1 function resulted in defective cytochrome c-dependent caspase activities and reduced apoptosis in embryo and in larval brain. Dapaf-1 activities were also involved in cell death induced by ectopic expression of reaper in the compound eye. These data suggest that Dapaf-1/cytochrome c-dependent cell death-inducing machinery is present in Drosophila, and the requirement of Dapaf-1/Apaf-1 in neural cell death is conserved through evolution.

MeSH Terms

Alternative Splicing Amino Acid Sequence Animals Apoptosis Apoptotic Protease-Activating Factor 1 Base Sequence Brain/cytology,embryology,metabolism Caenorhabditis elegans Proteins Calcium-Binding Proteins/chemistry,genetics Caspases/chemistry,metabolism Cell Line Cloning, Molecular Cytochrome c Group/metabolism Drosophila Proteins Drosophila melanogaster/cytology,embryology,enzymology,genetics Enzyme Activation Evolution, Molecular Eye/cytology,metabolism Helminth Proteins/chemistry,genetics Larva/cytology,metabolism Molecular Sequence Data Mutation/genetics Peptides/genetics,physiology Protein Binding Protein Isoforms/chemistry,genetics,metabolism Proteins/chemistry,genetics RNA, Messenger/analysis,genetics Sequence Homology, Amino Acid

Chemicals

Apoptotic Protease-Activating Factor 1 Caenorhabditis elegans Proteins Calcium-Binding Proteins Ced-4 protein, C elegans Cytochrome c Group Drosophila Proteins Helminth Proteins Peptides Protein Isoforms Proteins RNA, Messenger rpr protein, Drosophila Caspases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kanuka H

Department of Neuroscience, Osaka University Graduate School of Medicine, Japan.

Sawamoto K

Inohara N

Matsuno K

Okano H

Miura M

Article Info

Journal

Molecular cell

Abbr.

Mol Cell

ISSN

1097-2765

DOI

10.1016/s1097-2765(00)80386-x

Published

1999-11-00

Pages

757-69

Language

English

Region

United States

NLM ID

9802571

Subset

Databases

GENBANK

AB027531, AB027532

External Links

PubMed source DOI: 10.1016/s1097-2765(00)80386-x View journal details

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Control of the cell death pathway by Dapaf-1, a Drosophila Apaf-1/CED-4-related caspase activator.

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