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题目:: Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusion.
作者:: Strömberg(A),Ryttsén(F),Chiu(D T),Davidson(M),Eriksson(P S),Wilson(C F),Orwar(O),Zare(R N)
状态:: 发布时间2000-02-10 , 更新时间 2014-06-15
期刊:: Proc Natl Acad Sci U S A
摘要:: A method for cell-cell and cell-liposome fusion at the single-cell level is described. Individual cells or liposomes were first selected and manipulated either by optical trapping or by adhesion to a micromanipulator-controlled ultramicroelectrode. Spatially selective fusion of the cell-cell or cell-liposome pair was achieved by the application of a highly focused electric field through a pair of 5-micrometer o.d. carbon-fiber ultramicroelectrodes. The ability to fuse together single cells opens new possibilities in the manipulation of the genetic and cellular makeup of individual cells in a controlled manner. In the study of cellular networks, for example, the alteration of the biochemical identity of a selected cell can have a profound effect on the behavior of the entire network. Fusion of a single liposome with a target cell allows the introduction of the liposomal content into the cell interior as well as the addition of lipids and membrane proteins onto the cell surface. This cell-liposome fusion represents an approach to the manipulation of the cytoplasmic contents and surface properties of single cells. As an example, we have introduced a membrane protein (gamma-glutamyltransferase) reconstituted in liposomes into the cell plasma membrane.
语言:: eng
DOI:

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