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PMID: 10606647 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RNA aptamers for the MS2 bacteriophage coat protein and the wild-type RNA operator have similar solution behaviour.

Nucleic acids research ·Vol. 28 ·No. 2 ·2000-01-15 ·Pages 489-97

Parrott AM, Lago H, Adams CJ, Ashcroft AE, Stonehouse NJ, Stockley PG

Abstract

We have probed the effects of altering buffer conditions on the behaviour of two aptamer RNAs for the bacterio-phage MS2 coat protein using site-specific substitution of 2'-deoxy-2-aminopurine nucleotides at key adenosine positions. These have been compared to the wild-type operator stem-loop oligonucleotide, which is the natural target for the coat protein. The fluorescence emission spectra show a position and oligonucleotide sequence dependence which appears to reflect local conformational changes. These are largely similar between the differing oligonucleotides and deviations can be explained by the individual features of each sequence. Recognition by coat protein is enhanced, unaffected or decreased depending on the site of substitution, consistent with the known protein-RNA contacts seen in crystal structures of the complexes. These data suggest that the detailed conformational dynamics of aptamers and wild-type RNA ligands for the same protein target are remarkably similar.

MeSH Terms
Base Sequence Capsid Proteins/genetics Levivirus/genetics RNA, Viral/chemistry,genetics RNA-Binding Proteins/genetics Solutions Spectrometry, Fluorescence
Chemicals
Capsid Proteins RNA, Viral RNA-Binding Proteins Solutions
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Parrott A M
Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
Lago H
Adams C J
Ashcroft A E
Stonehouse N J
Stockley P G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
Published
2000-01-15
Pages
489-97
Language
English
Region
England
NLM ID
0411011
PMCID
PMC102504
Subset
IM
Grants
Wellcome Trust · United Kingdom
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