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PMID: 1060086 Published · ppublish English Journal Article

Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 10 ·1975-10-00 ·Pages 4028-32

Merlie JP, Sobel A, Changeux JP, Gros F

Abstract

Acetylcholine receptor, a component of the specialized muscle membrane, appears during the differentiation of embryonic myogenic cells in tissue culture. Demonstration of incorporation of the radioactive precursor L-[35S]methionine into purified receptor polypeptides is presented as evidence for its synthesis de novo. The identity of the purified radioactive species is established by cosedimentation of the [35S]receptor with [3H]alpha-toxin binding activity on sucrose gradients and by crossreaction with antiserum to purified acetylcholine receptor of Electrophorus electricus.

MeSH Terms
Acetylcholine/metabolism Actomyosin/biosynthesis Animals Binding Sites Binding, Competitive Cattle Cell Differentiation Cell Membrane/metabolism Cells, Cultured Creatine Kinase/metabolism Muscle Proteins/biosynthesis,immunology,metabolism Muscles/embryology,metabolism Precipitin Tests Protein Binding Receptors, Cholinergic Snake Venoms/metabolism
Chemicals
Muscle Proteins Receptors, Cholinergic Snake Venoms Actomyosin Creatine Kinase Acetylcholine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Merlie J P
Sobel A
Changeux J P
Gros F
References (22)
22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.10.4028
Published
1975-10-00
Pages
4028-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433131
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.10.4028 PMC Full Text View journal details
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