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PMID: 10588924 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Facilitation by 8-OH-DPAT of passive avoidance performance in rats after inactivation of 5-HT(1A) receptors.

British journal of pharmacology ·Vol. 128 ·No. 8 ·1999-12-00 ·Pages 1691-8

Otano A, García-Osta A, Ballaz S, Frechilla D, Del Río J

Abstract

1. Pretraining administration of 8-hydroxy-2-di-n-propylamino-tetralin (8-OH-DPAT 0.1 mg kg(-1)), a 5-HT(1A) receptor agonist, or buspirone (1 mg kg(-1)), a 5-HT(1A) receptor partial agonist, markedly impaired passive avoidance retention in rats 24 h later. The effect of 8-OH-DPAT was prevented by the 5-HT(1A) receptor antagonists, NAN-190 and WAY-100635, at doses without any intrinsic effect. 2. N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ 10 mg kg(-1)), an alkylating agent that inactivates different G-protein coupled receptors, impaired retention performance when given 48 h pretraining. The disruptive effect of EEDQ was reversed by 8-OH-DPAT or buspirone, given 30 min before training. 3. Non-specific actions did not account for 8-OH-DPAT-induced reversal of the EEDQ effect since no significant difference in locomotor activity or in pain threshold was found between rats receiving EEDQ or EEDQ+8-OH-DPAT. 4. When NAN-190 (1 mg kg(-1)) or WAY-100635 (0.5 mg kg(-1)) were given before 8-OH-DPAT to EEDQ-pretreated animals, the reversal by 8-OH-DPAT of EEDQ-induced retention impairment was still more pronounced. However, no EEDQ reversal by 8-OH-DPAT was found when 5-HT(1A) receptors were protected by WAY-100635 (10 mg kg(-1)) 30 min before EEDQ. 5. In the hippocampus of EEDQ-treated rats, 5-HT(7) receptors were less inactivated than 5-HT(1A) receptors and significant increases were found in 5-HT(1A) but not in 5-HT(7) receptor mRNA levels. Ritanserin and methiothepin (10 mg kg(-1) each), antagonists with higher affinity at 5-HT(7) than at 5-HT(1A) receptors, prevented the retention impairment induced by EEDQ but did not significantly protect against 5-HT(7) receptor inactivation. 6. The results indicate that the facilitatory effect of 8-OH-DPAT is not mediated through 5-HT(1A) receptors and suggest that other 8-OH-DPAT-sensitive receptors could be involved in the dual effect of 8-OH-DPAT on passive avoidance performance in rats.

MeSH Terms
8-Hydroxy-2-(di-n-propylamino)tetralin/pharmacology Alkylating Agents/pharmacology Animals Avoidance Learning/drug effects Brain Stem/drug effects,metabolism Hippocampus/drug effects,metabolism Male Quinolines/pharmacology Rats Rats, Wistar Receptors, Serotonin/drug effects,metabolism Receptors, Serotonin, 5-HT1 Serotonin Antagonists/pharmacology Serotonin Receptor Agonists/pharmacology
Chemicals
Alkylating Agents Quinolines Receptors, Serotonin Receptors, Serotonin, 5-HT1 Serotonin Antagonists Serotonin Receptor Agonists serotonin 7 receptor EEDQ 8-Hydroxy-2-(di-n-propylamino)tetralin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Otano A
Department of Pharmacology, School of Medicine, University of Navarra, Aptdo. 177, 31080-Pamplona, Spain.
García-Osta A
Ballaz S
Frechilla D
Del Río J
References (36)
36 references, click to expand
  1. The mechanism of irreversible adrenergic blockade by N-carbethoxydihydroquinolines--model studies with typical serine hydrolases.
    Biochem Pharmacol. 1969 May;18(5):1039-44 PMID: 5789772
  2. 5-HT1A and muscarinic acetylcholine receptors jointly regulate passive avoidance behavior.
    Eur J Pharmacol. 1994 Sep 1;262(1-2):77-90 PMID: 7813581
  3. Identification of presynaptic serotonin autoreceptors using a new ligand: 3H-PAT.
    Nature. 1983 Sep 8-14;305(5930):140-2 PMID: 6225026
  4. Quantitative autoradiographic mapping of serotonin receptors in the rat brain. I. Serotonin-1 receptors.
    Brain Res. 1985 Nov 4;346(2):205-30 PMID: 4052776
  5. Differential serotonin2 receptor recovery in mature and senescent rat brain after irreversible receptor modification: effect of chronic reserpine treatment.
    J Pharmacol Exp Ther. 1987 Oct;243(1):69-75 PMID: 2889827
  6. Buspirone impairment of performance of passive avoidance and spatial learning tasks in the rat.
    Psychopharmacology (Berl). 1990;100(3):393-8 PMID: 2315436
  7. Cloning, functional expression, and mRNA tissue distribution of the rat 5-hydroxytryptamine1A receptor gene.
    J Biol Chem. 1990 Apr 5;265(10):5825-32 PMID: 2156831
  8. 8-Hydroxy-2-(di-n-propylamino)tetralin, a 5-HT1A receptor agonist, impairs performance in a passive avoidance task.
    Eur J Pharmacol. 1992 Feb 11;211(2):227-34 PMID: 1535320
  9. Lack of apparent receptor reserve at postsynaptic 5-hydroxytryptamine1A receptors negatively coupled to adenylyl cyclase activity in rat hippocampal membranes.
    Mol Pharmacol. 1992 Jun;41(6):1066-72 PMID: 1352034
  10. 8-Hydroxy-2-(di-n-propylamino)tetralin impairs spatial learning in a water maze: role of postsynaptic 5-HT1A receptors.
    Br J Pharmacol. 1992 Mar;105(3):720-6 PMID: 1385752
  11. Comparison of 5-hydroxytryptamine1A-mediated hyperpolarization in CA1 and CA3 hippocampal pyramidal cells.
    J Pharmacol Exp Ther. 1992 Oct;263(1):350-9 PMID: 1403796
  12. Buspirone produces a dose-related impairment in spatial navigation.
    Pharmacol Biochem Behav. 1992 Sep;43(1):167-71 PMID: 1409800
  13. Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype.
    J Biol Chem. 1993 Aug 25;268(24):18200-4 PMID: 8394362
  14. 8-OH-DPAT impairs spatial but not visual learning in a water maze by stimulating 5-HT1A receptors in the hippocampus.
    Behav Brain Res. 1995 Feb;67(1):67-74 PMID: 7748502
  15. The pharmacology of VA21B7: an atypical 5-HT3 receptor antagonist with anxiolytic-like properties in animal models.
    Psychopharmacology (Berl). 1995 Jan;117(2):137-48 PMID: 7753959
  16. Characterisation of the binding of [3H]WAY-100635, a novel 5-hydroxytryptamine1A receptor antagonist, to rat brain.
    J Neurochem. 1995 Jun;64(6):2716-26 PMID: 7760052
  17. Characterization and distribution of putative 5-ht7 receptors in guinea-pig brain.
    Br J Pharmacol. 1995 May;115(1):107-16 PMID: 7647964
  18. Aplysia CREB2 represses long-term facilitation: relief of repression converts transient facilitation into long-term functional and structural change.
    Cell. 1995 Dec 15;83(6):979-92 PMID: 8521521
  19. Serotonergic modulation of cholinergic function in the central nervous system: cognitive implications.
    Neuroscience. 1995 Nov;69(1):1-41 PMID: 8637608
  20. Spatial memory deficits following stimulation of hippocampal 5-HT1B receptors in the rat.
    Eur J Pharmacol. 1995 Oct 24;285(3):221-8 PMID: 8575507
  21. A receptor autoradiographic and in situ hybridization analysis of the distribution of the 5-ht7 receptor in rat brain.
    Br J Pharmacol. 1996 Feb;117(4):657-66 PMID: 8646411
  22. Stereoselectivity of 8-OH-DPAT enantiomers at cloned human 5-HT1D receptor sites.
    Eur J Pharmacol. 1996 Apr 4;300(1-2):137-9 PMID: 8741179
  23. Time-course of recovery of 5-HT1A receptors and changes in 5-HT1A receptor mRNA after irreversible inactivation with EEDQ.
    Brain Res Mol Brain Res. 1996 Jun;38(2):233-42 PMID: 8793111
  24. Age-related assessment of central 5-HT1A receptors following irreversible inactivation by N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ).
    Brain Res. 1996 Jul 22;728(1):130-4 PMID: 8864308
  25. Differential regulation by methylenedioxymethamphetamine of 5-hydroxytryptamine1A receptor density and mRNA expression in rat hippocampus, frontal cortex, and brainstem: the role of corticosteroids.
    J Neurochem. 1997 Mar;68(3):1099-105 PMID: 9048755
  26. Contrasting effects of systemic and intracerebral infusions of the 5-HT1A receptor agonist 8-OH-DPAT on spatial short-term working memory in rats.
    Behav Brain Res. 1997 Mar;84(1-2):247-58 PMID: 9079789
  27. The effects of novel, selective 5-hydroxytryptamine (5-HT)4 receptor ligands in rat spatial navigation.
    Neuropharmacology. 1997 Apr-May;36(4-5):689-96 PMID: 9225295
  28. Enhancement and depression of spinal reflexes by 8-hydroxy-2-(di-n-propylamino)tetralin in the decerebrated and spinalized rabbit: involvement of 5-HT1A- and non-5-HT1A-receptors.
    Br J Pharmacol. 1997 Oct;122(4):631-8 PMID: 9375958
  29. Effects of the serotonin agonists 8-OH-DPAT, buspirone, and DOI on water maze performance.
    Pharmacol Biochem Behav. 1998 Mar;59(3):729-35 PMID: 9512079
  30. Agonist-induced desensitization of adenylyl cyclase activity mediated by 5-hydroxytryptamine7 receptors in rat frontocortical astrocytes.
    Brain Res. 1998 Feb 16;784(1-2):57-62 PMID: 9518551
  31. Analysis of the 5-HT1A receptor involvement in passive avoidance in the rat.
    Br J Pharmacol. 1998 Oct;125(3):499-509 PMID: 9806333
  32. On the role of the hippocampus in learning and memory in the rat.
    Behav Neural Biol. 1993 Jul;60(1):9-26 PMID: 8216164
  33. Inhibitory modulation of long-term potentiation via the 5-HT1A receptor in slices of the rat hippocampal dentate gyrus.
    Brain Res. 1993 Jun 11;613(2):326-30 PMID: 8186986
  34. Molecular biology of 5-HT receptors.
    Neuropharmacology. 1994 Mar-Apr;33(3-4):275-317 PMID: 7984267
  35. Differential effects of N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) on various 5-HT receptor binding sites in the rat brain.
    Neuropharmacology. 1994 Mar-Apr;33(3-4):423-31 PMID: 7984280
  36. An autoradiographic analysis of the differential ascending projections of the dorsal and median raphe nuclei in the rat.
    J Comp Neurol. 1978 Jun 1;179(3):641-67 PMID: 565370
Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1038/sj.bjp.0702974
Published
1999-12-00
Pages
1691-8
Language
English
Region
England
NLM ID
7502536
PMCID
PMC1571811
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.bjp.0702974 PMC Full Text View journal details
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