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PMID: 10579699 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Reduction of sialic acid O-acetylation in human colonic mucins in the adenoma-carcinoma sequence.

Glycoconjugate journal ·Vol. 16 ·No. 6 ·1999-06-00 ·Pages 307-17

Corfield AP, Myerscough N, Warren BF, Durdey P, Paraskeva C, Schauer R

Abstract

The oligo-O-acetylation of sialic acids found in normal colonic mucins is greatly reduced in colorectal cancer. Mucins prepared from cancer tissue in adenocarcinoma showed this reduction, while normal O-acetylation was detected in resection margin and control cases and total mucin sialic acid content was significantly decreased in cancer vs. control samples. A reduction of the O-acetyl transferase activity catalysing the O-acetylation reaction was also found. A series of cultured human colorectal cell lines derived from the same premalignant adenomatous line, and representative of the adenoma-carcinoma sequence were examined and revealed a depletion of oligo-O-acetylation in the original diploid premalignant line, re-expression in a further premalignant line and reduction in malignant mucinous and adenocarcinoma cell lines. Reduction of sialic acid O-acetylation appears as an early event in the process of malignant transformation in human colorectal cancer.

MeSH Terms
Acetylation Acetyltransferases/metabolism Adenocarcinoma/enzymology,metabolism Chromatography, High Pressure Liquid Chromatography, Thin Layer Colorectal Neoplasms/enzymology,metabolism Epitopes Humans Mucins/chemistry,metabolism N-Acetylneuraminic Acid/chemistry,metabolism Tumor Cells, Cultured
Chemicals
Epitopes Mucins Acetyltransferases N-Acetylneuraminic Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Corfield A P
University Department of Medicine, Bristol Royal Infirmary, UK. Tony.Corfield@bristol.ac.uk
Myerscough N
Warren B F
Durdey P
Paraskeva C
Schauer R
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Article Info
Journal
Glycoconjugate journal
Abbr.
Glycoconj J
ISSN
0282-0080
DOI
10.1023/a:1007026314792
Email
tony.corfield@bristol.ac.uk
Published
1999-06-00
Pages
307-17
Language
English
Region
United States
NLM ID
8603310
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1007026314792 View journal details
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