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PMID: 10567263 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Specific lipopolysaccharide found in cystic fibrosis airway Pseudomonas aeruginosa.

Science (New York, N.Y.) ·Vol. 286 ·No. 5444 ·1999-11-19 ·Pages 1561-5

Ernst RK, Yi EC, Guo L, Lim KB, Burns JL, Hackett M, Miller SI

Abstract

Cystic fibrosis (CF) patients develop chronic airway infections with Pseudomonas aeruginosa (PA). Pseudomonas aeruginosa synthesized lipopolysaccharide (LPS) with a variety of penta- and hexa-acylated lipid A structures under different environmental conditions. CF patient PA synthesized LPS with specific lipid A structures indicating unique recognition of the CF airway environment. CF-specific lipid A forms containing palmitate and aminoarabinose were associated with resistance to cationic antimicrobial peptides and increased inflammatory responses, indicating that they are likely to be involved in airway disease.

MeSH Terms

Acylation Antimicrobial Cationic Peptides Arabinose/analogs & derivatives,analysis,metabolism Bacterial Proteins/genetics,physiology Cells, Cultured Cystic Fibrosis/complications,microbiology Drug Resistance, Microbial Humans Infant Interleukin-8/biosynthesis Lipid A/biosynthesis,chemistry Lipopolysaccharides/chemistry,immunology Magnesium/pharmacology Mutation Palmitates/analysis,metabolism Peptides/pharmacology Polymyxins/pharmacology Pseudomonas Infections/microbiology Pseudomonas aeruginosa/drug effects,genetics,metabolism,pathogenicity Respiratory System/microbiology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Virulence

Chemicals

Antimicrobial Cationic Peptides Bacterial Proteins C18G peptide Interleukin-8 Lipid A Lipopolysaccharides Palmitates Peptides PhoQ protein, Bacteria Polymyxins aminoarabinose PhoP protein, Bacteria Arabinose Magnesium

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Ernst R K

Department of Medicine, University of Washington, Seattle, WA 98195, USA.

Yi E C

Guo L

Lim K B

Burns J L

Hackett M

Miller S I

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.286.5444.1561

Published

1999-11-19

Pages

1561-5

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

PHS HHS · R21 R13400 · United States

NHLBI NIH HHS · R55 HL 48888 · United States

External Links

PubMed source DOI: 10.1126/science.286.5444.1561 View journal details

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Specific lipopolysaccharide found in cystic fibrosis airway Pseudomonas aeruginosa.

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