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PMID: 1056038 Published · ppublish English Journal Article

Cytoplasmic control of nuclear DNA synthesis during early development of Xenopus laevis: a cell-free assay.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2437-41

Benbow RM, Ford CC

Abstract

Nuclei isolated from nondividing cells were induced to synthesize DNA by incubation with cytoplasm from early embryos of Xenopus laevis. Numerous replication eyes were formed in the nuclear DNA molecules, and high levels of [3-H]dTTP were incorporated. With this assay a protein(s) which appears to initiate DNA synthesis was found at high levels in the cytoplasm of eggs, blastulae, or gastrulae, but only at low levels in the cytoplasm of oocytes, hatched embryos, or adult tissues.

MeSH Terms
Animals Anura Autoradiography Cell Nucleus/drug effects,metabolism Cell-Free System Chromatography, Gel Cytoplasm DNA/biosynthesis DNA Replication/drug effects Embryo, Nonmammalian/ultrastructure Embryonic Development Female Kinetics Liver/drug effects,metabolism,ultrastructure Microscopy, Electron Ovum Proteins/isolation & purification,pharmacology Xenopus/embryology,growth & development
Chemicals
Proteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Benbow R M
Ford C C
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2437
Published
1975-06-00
Pages
2437-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432774
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.6.2437 PMC Full Text View journal details
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