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PMID: 10559447 Published · ppublish English Journal Article

Positional specificity of a phospholipase A activity induced by wounding, systemin, and oligosaccharide elicitors in tomato leaves

The Plant cell ·Vol. 11 ·No. 11 ·1999-11-00 ·Pages 2249-60

Narvaez-Vasquez J, Florin-Christensen J, Ryan CA

Abstract

Phospholipase A (PLA) activity, as measured by the accumulation of (14)C-lysophosphatidylcholine in leaves of tomato plants, increased rapidly and systemically in response to wounding. The increase in PLA activity in the systemic unwounded leaves was biphasic in wild-type tomato plants, peaking at 15 min and again at 60 min, but the second peak of activity was absent in transgenic prosystemin antisense plants. Supplying young excised tomato plants with the polypeptide hormone systemin also caused (14)C-lysophosphatidylcholine to increase to levels similar to those induced by wounding, but the increase in activity persisted for >2 hr. Antagonists of systemin blocked both the release of (14)C-lysophosphatidylcholine and the accumulation of defense proteins in response to systemin. (14)C-lysophosphatidylcholine levels did not increase in response to jasmonic acid. Chemical acylation of the lysophosphatidylcholine produced by wounding, systemin, and oligosaccharide elicitors followed by enzymatic hydrolysis with lipases of known specificities demostrated that the lysophosphatidylcholine is generated by a PLA with specificity for the sn-2 position.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Narvaez-Vasquez
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA.
Florin-Christensen
Ryan
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.11.11.2249
Published
1999-11-00
Pages
2249-60
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144127
External Links
PubMed source DOI: 10.1105/tpc.11.11.2249 PMC Full Text View journal details
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