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PMID: 10559370 Published · ppublish English Journal Article

Quantitation of latent varicella-zoster virus and herpes simplex virus genomes in human trigeminal ganglia.

Journal of virology ·Vol. 73 ·No. 12 ·1999-12-00 ·Pages 10514-8

Pevenstein SR, Williams RK, McChesney D, Mont EK, Smialek JE, Straus SE

Abstract

Using real-time fluorescence PCR, we quantitated the numbers of copies of latent varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) genomes in 15 human trigeminal ganglia. Eight (53%) and 1 (7%) of 15 ganglia were PCR positive for HSV-1 or -2 glycoprotein G genes, with means of 2,902 +/- 1,082 (standard error of the mean) or 109 genomes/10(5) cells, respectively. Eleven of 14 (79%) to 13 of 15 (87%) of the ganglia were PCR positive for VZV gene 29, 31, or 62. Pooling of the results for the three VZV genes yielded a mean of 258 +/- 38 genomes/10(5) ganglion cells. These levels of latent viral genome loads have implications for virus distribution in and reactivation from human sensory ganglia.

MeSH Terms
Adolescent Adult Aged Chickenpox/pathology,virology DNA, Viral Female Genome, Viral Herpes Genitalis/pathology,virology Herpes Simplex/pathology,virology Herpesvirus 1, Human/genetics,isolation & purification Herpesvirus 2, Human/genetics,isolation & purification Herpesvirus 3, Human/genetics,isolation & purification Humans Immediate-Early Proteins/genetics Male Middle Aged Polymerase Chain Reaction/methods Trans-Activators/genetics Trigeminal Ganglion/virology Viral Envelope Proteins/genetics Viral Load Virus Latency
Chemicals
DNA, Viral IE62 protein, Human herpesvirus 3 Immediate-Early Proteins Trans-Activators Viral Envelope Proteins glycoprotein G, herpes simplex virus type 2 glycoprotein gG-1, herpes simplex virus type 1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pevenstein S R
Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Williams R K
McChesney D
Mont E K
Smialek J E
Straus S E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.12.10514-10518.1999
Published
1999-12-00
Pages
10514-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC113107
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.73.12.10514-10518.1999 PMC Full Text View journal details
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