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PMID: 1055377 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Crystallization of nerve growth factor from mouse submaxillary glands.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 3 ·1975-03-00 ·Pages 777-9

Wlodawer A, Hodgson KO, Shooter EM

Abstract

Crystals of the nerve growth factor protein were grown by vapor diffusion from ethanol solution. The crystals are hexagonal, belonging to space group P622 (or its enantiomorph) with a equals 56.1 A, c equals 181.4 A, and V equals 494,400 A. The unit cell contains six molecules of dimeric protein and thus has one monomer per asymmetric unit. The diffraction pattern extends to at least 2.7 A, indicating that this crystal form is suitable for structural analysis to near-atomic resolution.

MeSH Terms
Animals Crystallization Male Mice Nerve Growth Factors/isolation & purification Protein Conformation Submandibular Gland/analysis X-Ray Diffraction
Chemicals
Nerve Growth Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wlodawer A
Hodgson K O
Shooter E M
References (13)
13 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.3.777
Published
1975-03-00
Pages
777-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432402
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.3.777 PMC Full Text View journal details
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