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PMID: 1054843 Published · ppublish English Comparative Study Journal Article

O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 2 ·1975-02-00 ·Pages 619-22

Benson JR, Hare PE

Abstract

O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers.

MeSH Terms
Aldehydes Amines/analysis Amino Acids/analysis Benzofurans Fluorescence Globins/analysis Mercaptoethanol Ninhydrin Peptides/analysis Phthalic Acids/analogs & derivatives Spiro Compounds
Chemicals
Aldehydes Amines Amino Acids Benzofurans Peptides Phthalic Acids Spiro Compounds Mercaptoethanol Globins Ninhydrin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Benson J R
Hare P E
References (10)
10 references, click to expand
  1. Single column analysis of amino acids in protein hydrolysates utilizing the fluorescamine reaction.
    Anal Biochem. 1973 Nov;56(1):121-8 PMID: 4797194
  2. Lysosomal peptidase measurement by sensitive fluorometric amino acid analysis.
    Anal Biochem. 1973 Nov;56(1):140-8 PMID: 4764684
  3. Determination of hydroxyproline in fluorometric amino acid analysis with fluorescamine.
    Anal Biochem. 1973 Dec;56(2):610-5 PMID: 4765649
  4. Column chromatography of amino acids with fluorescence detection.
    J Chromatogr. 1973 Aug 29;83:353-6 PMID: 4733857
  5. Total fluorometric amino acid analysis using fluorescamine.
    Arch Biochem Biophys. 1973 Jul;157(1):177-82 PMID: 4716953
  6. Ion-exchange chromatography in lunar organic analysis.
    Space Life Sci. 1972 Oct;3(4):354-9 PMID: 4650294
  7. Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.
    Science. 1972 Nov 24;178(4063):871-2 PMID: 5085985
  8. Fluorescence reaction for amino acids.
    Anal Chem. 1971 Jun;43(7):880-2 PMID: 5576608
  9. A new sensitive, rapid fluorescence technique for the determination of proteins in gel electrophoresis and in solution.
    Anal Biochem. 1973 Jul;54(1):102-14 PMID: 4125254
  10. Removal of a Fluoram 1-positive impurity from hydrochloric acid.
    Anal Biochem. 1974 Sep;61(1):302-4 PMID: 4415552
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.2.619
Published
1975-02-00
Pages
619-22
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432365
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.2.619 PMC Full Text View journal details
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