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PMID: 10543773 Published · ppublish English Journal Article

Molecular characterization of rpoB mutations conferring cross-resistance to rifamycins on methicillin-resistant Staphylococcus aureus.

Antimicrobial agents and chemotherapy ·Vol. 43 ·No. 11 ·1999-11-00 ·Pages 2813-6

Wichelhaus TA, Schäfer V, Brade V, Böddinghaus B

Abstract

Mutations of the rpoB gene conferring resistance to rifampin were analyzed in 40 methicillin-resistant Staphylococcus aureus isolates obtained from six countries. Interestingly, the majority of clinical isolates showed multiple mutations within rpoB. The amino acid substitution 481His-->Asn was the most prevalent one, capable of conferring low-level resistance on its own. Cross-resistance to rifampin, rifabutin, and rifapentine was demonstrated for all mutants identified. The level of resistance to rifamycins correlated with both the mutation position and type of amino acid substitution.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/pharmacology DNA, Bacterial/analysis,genetics DNA-Directed RNA Polymerases Drug Resistance, Microbial Electrophoresis, Polyacrylamide Gel Escherichia coli/drug effects,genetics Genotype Humans Methicillin Resistance/genetics Microbial Sensitivity Tests Molecular Sequence Data Multigene Family/genetics Plant Proteins/genetics Rifamycins/pharmacology Staphylococcal Infections/microbiology Staphylococcus aureus/drug effects,genetics
Chemicals
Anti-Bacterial Agents DNA, Bacterial Plant Proteins Rifamycins RpoB protein, Oryza sativa DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wichelhaus T A
Institute of Medical Microbiology, University Hospital of Frankfurt, Frankfurt am Main, Germany.
Schäfer V
Brade V
Böddinghaus B
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.43.11.2813
Published
1999-11-00
Pages
2813-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC89569
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.43.11.2813 PMC Full Text View journal details
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