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PMID: 10531281 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Binding of pili from uropathogenic Escherichia coli to membranes secreted by human colonocytes and enterocytes.

Infection and immunity ·Vol. 67 ·No. 11 ·1999-11-00 ·Pages 6161-3

Goetz GS, Mahmood A, Hultgren SJ, Engle MJ, Dodson K, Alpers DH

Abstract

PapG adhesins mediate the binding of uropathogenic Escherichia coli. Although receptors for these adhesins have not been demonstrated in intestinal epithelia, the colonic microflora includes strains of uropathogenic E. coli. We now report that surfactant-like particles secreted by the human intestine contain receptors for PapG adhesins and may provide an intestinal habitat for uropathogenic bacteria.

MeSH Terms
Adhesins, Escherichia coli/physiology Bacterial Adhesion Colon/microbiology Enterocytes/microbiology Escherichia coli/physiology Fimbriae Proteins Fimbriae, Bacterial/physiology Humans Urinary Tract Infections/microbiology
Chemicals
Adhesins, Escherichia coli PapG protein, E coli Fimbriae Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Goetz G S
Department of Internal Medicine, Division of Gastroenterology, Washington University School of Medicine, St. Louis, Missouri, USA.
Mahmood A
Hultgren S J
Engle M J
Dodson K
Alpers D H
References (21)
21 references, click to expand
  1. Adhesion to normal human uroepithelial cells of Escherichia coli from children with various forms of urinary tract infection.
    J Pediatr. 1978 Sep;93(3):398-403 PMID: 357693
  2. HOST-PARASITE INTERACTION IN PATIENTS WITH INFECTIONS DUE TO ESCHERICHIA COLI. I. THE SEROGROUPING OF E. COLI FROM INTESTINAL AND EXTRAINTESTINAL SOURCES.
    J Clin Invest. 1964 Dec;43:2377-85 PMID: 14234834
  3. The PapG protein is the alpha-D-galactopyranosyl-(1----4)-beta-D-galactopyranose-binding adhesin of uropathogenic Escherichia coli.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5898-902 PMID: 2886993
  4. Quantitative determination of the lectin binding capacity of small intestinal brush-border membrane. An enzyme linked lectin sorbent assay (ELLSA).
    Biochim Biophys Acta. 1987 Dec 11;905(2):371-5 PMID: 3318929
  5. Attachment of Escherichia coli via mannose- or Gal alpha 1----4Gal beta-containing receptors to human colonic epithelial cells.
    Infect Immun. 1988 Oct;56(10):2531-7 PMID: 2901402
  6. Glycolipids of human large intestine: difference in glycolipid expression related to anatomical localization, epithelial/non-epithelial tissue and the ABO, Le and Se phenotypes of the donors.
    Biochimie. 1988 Nov;70(11):1565-74 PMID: 3149523
  7. The PapG adhesin of uropathogenic Escherichia coli contains separate regions for receptor binding and for the incorporation into the pilus.
    Proc Natl Acad Sci U S A. 1989 Jun;86(12):4357-61 PMID: 2567514
  8. Host-specificity of uropathogenic Escherichia coli depends on differences in binding specificity to Gal alpha 1-4Gal-containing isoreceptors.
    EMBO J. 1990 Jun;9(6):2001-10 PMID: 1693334
  9. Saccharide orientation at the cell surface affects glycolipid receptor function.
    Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9340-4 PMID: 1681550
  10. Immunoglobulin-like PapD chaperone caps and uncaps interactive surfaces of nascently translocated pilus subunits.
    Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10586-90 PMID: 1683704
  11. Resident colonic Escherichia coli strains frequently display uropathogenic characteristics.
    J Infect Dis. 1992 Jan;165(1):46-52 PMID: 1727897
  12. P pili in uropathogenic E. coli are composite fibres with distinct fibrillar adhesive tips.
    Nature. 1992 Mar 19;356(6366):252-5 PMID: 1348107
  13. Characterization of proteins in rat and human intestinal surfactant-like particles.
    Arch Biochem Biophys. 1993 Jan;300(1):280-6 PMID: 8424664
  14. Pilus and nonpilus bacterial adhesins: assembly and function in cell recognition.
    Cell. 1993 Jun 4;73(5):887-901 PMID: 8098994
  15. P fimbriae of uropathogenic Escherichia coli as multifunctional adherence organelles.
    Zentralbl Bakteriol. 1993 Apr;278(2-3):229-37 PMID: 8102269
  16. Structural basis of pilus subunit recognition by the PapD chaperone.
    Science. 1993 Nov 19;262(5137):1234-41 PMID: 7901913
  17. A possible role for rat intestinal surfactant-like particles in transepithelial triacylglycerol transport.
    J Clin Invest. 1994 Jan;93(1):70-80 PMID: 8282824
  18. Appearance of surfactant-like particles in apical medium of Caco-2 cells may occur via tight junctions.
    Am J Physiol. 1995 Jun;268(6 Pt 1):C1401-13 PMID: 7611359
  19. Isolation and characterization of surfactant-like particles in rat and human colon.
    Am J Physiol. 1997 Mar;272(3 Pt 1):G425-34 PMID: 9124562
  20. Discovery of disseminated J96-like strains of uropathogenic Escherichia coli O4:H5 containing genes for both PapG(J96) (class I) and PrsG(J96) (class III) Gal(alpha1-4)Gal-binding adhesins.
    J Infect Dis. 1997 Apr;175(4):983-8 PMID: 9086165
  21. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate.
    Infect Immun. 1981 Sep;33(3):933-8 PMID: 6116675
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.67.11.6161-6163.1999
Published
1999-11-00
Pages
6161-3
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC97007
Subset
IM
Grants
NIDDK NIH HHS · DK 14038 · United States
NIAID NIH HHS · R01 AI029549 · United States
NIAID NIH HHS · R37 AI029549 · United States
NIDDK NIH HHS · R01 DK051406 · United States
NIDDK NIH HHS · DK51406 · United States
NIAID NIH HHS · AI29549 · United States
NIDDK NIH HHS · F32 DK009329 · United States
External Links
PubMed source DOI: 10.1128/IAI.67.11.6161-6163.1999 PMC Full Text View journal details
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