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PMID: 1052169 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Separation of human hemoglobins by DEAE-cellulose chromatography using glycine-KCN-NaC1 developers.

Hemoglobin ·Vol. 1 ·No. 1 ·1976-00-00 ·Pages 27-44

Abraham EC, Reese A, Stallings M, Huisman TH

Abstract

This chromatographic procedure uses DEAE-cellulose as ion exchanger and glycine-KCN-NaC1 solutions as developers. Blood samples from several adults and newborn infants with alpha, beta, delta, or gamma chains variants have been analysed. The hemoglobins are eluted as compact and symmetrical zones, and the separation of many hemoglobin types is greatly improved. The procedure is relatively fast, simple, and inexpensive.

MeSH Terms

Adult Chromatography, DEAE-Cellulose Cyanides/pharmacology Genetic Variation Georgia Glycine/pharmacology Hemoglobin A/isolation & purification Hemoglobin J/isolation & purification Hemoglobin, Sickle/isolation & purification Hemoglobins/isolation & purification Humans Infant, Newborn Pennsylvania Sodium Chloride/pharmacology

Chemicals

Cyanides Hemoglobin, Sickle Hemoglobins Sodium Chloride Hemoglobin J Hemoglobin A Glycine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Abraham E C

Reese A

Stallings M

Huisman T H

Article Info

Journal

Hemoglobin

Abbr.

Hemoglobin

ISSN

0363-0269

DOI

10.3109/03630267609031020

Published

1976-00-00

Pages

27-44

Language

English

Region

England

NLM ID

7705865

Subset

External Links

PubMed source DOI: 10.3109/03630267609031020 View journal details

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Separation of human hemoglobins by DEAE-cellulose chromatography using glycine-KCN-NaC1 developers.

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