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PMID: 10521527 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The role of opaque2 in the control of lysine-degrading activities in developing maize endosperm.

The Plant cell ·Vol. 11 ·No. 10 ·1999-10-00 ·Pages 1981-94

Kemper EL, Neto GC, Papes F, Moraes KC, Leite A, Arruda P

Abstract

We have isolated a cDNA clone, designated ZLKRSDH, encoding the bifunctional enzyme lysine-ketoglutarate reductase/saccharopine dehydrogenase (LKR/SDH) from maize. The predicted polypeptide has an N-terminal LKR domain and a C-terminal SDH domain that are similar to the yeast LYS1 and LYS9 monofunctional proteins, respectively. The maize LKR/SDH protein is located in the cytoplasm of subaleurone endosperm cell layers. Transcripts and polypeptides as well as enzyme activities showed an upregulation and downregulation during endosperm development. The developmental expression of ZLKRSDH was examined in normal and opaque2 seeds. In the mutant endosperm, mRNA levels were reduced by >90%, with concomitant reductions in polypeptide levels and LKR/SDH activity. These results suggest that lysine levels in the endosperm are likely to be controlled at the transcriptional level by the Opaque2 transcription factor.

MeSH Terms
Amino Acid Sequence Cytosol/enzymology DNA, Complementary DNA-Binding Proteins/physiology Hydrolysis Lysine/metabolism Molecular Sequence Data Plant Proteins RNA, Messenger/genetics,metabolism Saccharopine Dehydrogenases/genetics,metabolism Sequence Homology, Amino Acid Transcription Factors/physiology Zea mays/metabolism
Chemicals
DNA, Complementary DNA-Binding Proteins Plant Proteins RNA, Messenger Transcription Factors opaque-2 protein, Zea mays Saccharopine Dehydrogenases saccharopine dehydrogenase (NADP+, lysine-forming) Lysine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kemper E L
Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil.
Neto G C
Papes F
Moraes K C
Leite A
Arruda P
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.11.10.1981
Published
1999-10-00
Pages
1981-94
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144114
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.11.10.1981 PMC Full Text View journal details
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