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PMID: 10492129 Published · ppublish English Journal Article

Bombesin ameliorates colonic damage in experimental colitis.

Digestive diseases and sciences ·Vol. 44 ·No. 8 ·1999-08-00 ·Pages 1531-8

Güllüoğlu BM, Kurtel H, Güllüoğlu MG, Aktan AO, Yeğen BC, Dizdaroğlu F, Yalin R, Yeğen BC

Abstract

In the present study we investigated the possible therapeutic effects of bombesin on an experimentally induced colitis model in rats. Inflammation of the colon was induced by a single intracolonic administration of 30 mg of 2,4,6-trinitrobenzene sulfonic acid (TNBS) at 8 cm from the anus. Immediately after the induction of colitis, some rats were given bombesin (10 microg/kg; subcutaneously) three times a day for 14 days, while another group received vehicle treatment. On day 14, the rats were decapitated and plasma carbonyl content and tissue myeloperoxidase level, as an index of granulocyte infiltration into intestinal tissue, were determined in order to obtain an objective evaluation of colonic injury. In the colitis group, increased macroscopic damage score, elevated MPO level and high plasma carbonyl content, together with the microscopic appearance revealed severe inflammatory changes resembling IBD. Bombesin treatment attenuated the TNBS-induced colonic damage and stimulated histopathologically apparent mucosal proliferation, suggesting that bombesin may play a role in protecting gut integrity.

MeSH Terms
Animals Bombesin/pharmacology Colitis/blood,enzymology,metabolism,pathology Colon/drug effects,enzymology,pathology Gastrointestinal Transit/drug effects Male Oxidation-Reduction/drug effects Proteins/metabolism Rats Rats, Wistar
Chemicals
Proteins Bombesin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Güllüoğlu B M
Marmara University School of Medicine, Department of General Surgery, Turkey.
Kurtel H
Güllüoğlu M G
Aktan A O
Yeğen B C
Dizdaroğlu F
Yalin R
Yeğen B C
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Article Info
Journal
Digestive diseases and sciences
Abbr.
Dig Dis Sci
ISSN
0163-2116
DOI
10.1023/a:1026646523284
Published
1999-08-00
Pages
1531-8
Language
English
Region
United States
NLM ID
7902782
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1026646523284 View journal details
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