Dynamics of transient pores in stretched vesicles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 19 ·1999-09-14 ·Pages 10591-6

Sandre O, Moreaux L, Brochard-Wyart F

Abstract

We image macroscopic transient pores in mechanically stretched giant vesicles. Holes open above a critical radius r(c1), grow up to a radius r(c2), and close. We interpret the upper limit r(c2) by a relaxation of the membrane tension as the holes expand. The closing of the holes is caused by a further relaxation of the surface tension when the internal liquid leaks out. A dynamic model fits our data for the growth and closure of pores.

MeSH Terms

Cell Membrane Permeability Electrophysiology Glycerol/pharmacology Lipid Bilayers/chemistry Membrane Potentials Membranes, Artificial Models, Biological Models, Theoretical Polylysine/chemistry Surface Properties Time Factors

Chemicals

Lipid Bilayers Membranes, Artificial Polylysine Glycerol

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sandre O

Laboratoire Physico-Chimie Curie-Unité Mixte de Recherche 168 Centre National de la Recherche Scientifique/Institut Curie, 11, rue Pierre et Marie Curie 75248 Paris CEDEX 05, France. Sandre@curie.fr

Moreaux L

Brochard-Wyart F

References (11)

11 references, click to expand

Targeted gene transfer into hepatoma cells with lipopolyamine-condensed DNA particles presenting galactose ligands: a stage toward artificial viruses.
Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1744-8 PMID: 7878052
Opening-up of liposomal membranes by talin.
Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1026-31 PMID: 9448279
Nonviral gene therapy: the promise of genes as pharmaceutical products.
Hum Gene Ther. 1995 Sep;6(9):1129-44 PMID: 8527471
Entropy-driven tension and bending elasticity in condensed-fluid membranes.
Phys Rev Lett. 1990 Apr 23;64(17):2094-2097 PMID: 10041575
Dynamical fluctuations of droplet microemulsions and vesicles.
Phys Rev A Gen Phys. 1987 Nov 1;36(9):4371-4379 PMID: 9899393
Viscous bursting of suspended films.
Phys Rev Lett. 1995 Nov 20;75(21):3886-3889 PMID: 10059756
Drug delivery: piercing vesicles by their adsorption onto a porous medium.
Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9590-2 PMID: 7568178
Microinterferometry: Three-Dimensional Reconstruction of Surface Microtopography for Thin-Film and Wetting Studies by Reflection Interference Contrast Microscopy (RICM).
Appl Opt. 1998 Oct 10;37(29):6892-905 PMID: 18301506
The life and death of "Bare" viscous bubbles
Science. 1998 Mar 13;279(5357):1704-7 PMID: 9497285
Engineering cell shape and function.
Science. 1994 Apr 29;264(5159):696-8 PMID: 8171320
Dynamically stabilized pores in bilayer membranes.
Biophys J. 1997 May;72(5):2211-6 PMID: 9129823

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.96.19.10591

sandre@curie.fr

Published

1999-09-14

Pages

10591-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC17927

Subset

Corrections

CommentIn

PMID: 10485856

External Links

PubMed source DOI: 10.1073/pnas.96.19.10591 PMC Full Text View journal details

Analysis Services

Dynamics of transient pores in stretched vesicles.

Contact

WeChat Official Account