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PMID: 10482534 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Role of CCAA nucleotide repeats in regulation of hemoglobin and hemoglobin-haptoglobin binding protein genes of Haemophilus influenzae.

Journal of bacteriology ·Vol. 181 ·No. 18 ·1999-09-00 ·Pages 5865-70

Ren Z, Jin H, Whitby PW, Morton DJ, Stull TL

Abstract

Haemophilus influenzae utilizes hemoglobin and hemoglobin-haptoglobin as heme sources. The H. influenzae hemoglobin- and hemoglobin-haptoglobin binding protein genes, hgpA, hgpB, and hgpC, contain lengths of tetrameric CCAA repeats. Using an hgpA-lacZ translational gene fusion, we demonstrate phase-variable expression of lacZ associated with alteration in the length of the CCAA repeat region.

MeSH Terms
Bacterial Outer Membrane Proteins/biosynthesis,genetics Bacterial Proteins Base Sequence Carrier Proteins/biosynthesis,genetics DNA Primers Gene Expression Regulation, Bacterial Genes, Bacterial Haemophilus influenzae/genetics Haptoglobins/metabolism Hemoglobins/metabolism Microsatellite Repeats/genetics Molecular Sequence Data Protein Biosynthesis Recombinant Fusion Proteins/biosynthesis Restriction Mapping beta-Galactosidase/genetics
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins DNA Primers Haptoglobins Hemoglobins HgpC protein, Haemophilus influenzae Recombinant Fusion Proteins haptoglobin-hemoglobin complex hemoglobin-binding protein, bacteria beta-Galactosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ren Z
Departments of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA.
Jin H
Whitby P W
Morton D J
Stull T L
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17 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.18.5865-5870.1999
Published
1999-09-00
Pages
5865-70
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94113
Subset
IM
Grants
NIAID NIH HHS · R01 AI029611 · United States
NIAID NIH HHS · R56 AI029611 · United States
NIAID NIH HHS · AI29611 · United States
External Links
PubMed source DOI: 10.1128/JB.181.18.5865-5870.1999 PMC Full Text View journal details
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