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PMID: 10477258 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells.

The Biochemical journal ·Vol. 342 Pt 3 ·1999-09-15 ·Pages 497-501

Duncan RR, Don-Wauchope AC, Tapechum S, Shipston MJ, Chow RH, Estibeiro P

Abstract

Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

MeSH Terms
Adrenal Medulla/cytology Animals Cattle Chromaffin Cells/cytology,virology Culture Media Electrophysiology Exocytosis Gene Transfer Techniques Green Fluorescent Proteins Luminescent Proteins/genetics,metabolism Mice Microscopy, Confocal Polymerase Chain Reaction Semliki forest virus/genetics
Chemicals
Culture Media Luminescent Proteins Green Fluorescent Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Duncan R R
Membrane Biology Group, Biomedical Sciences, University of Edinburgh Medical School, Teviot Place, Edinburgh, EH8 9XD, U.K.
Don-Wauchope A C
Tapechum S
Shipston M J
Chow R H
Estibeiro P
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1999-09-15
Pages
497-501
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1220488
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source PMC Full Text View journal details
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