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PMID: 10471835 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dissociation of the eukaryotic initiation factor-4E/4E-BP1 complex involves phosphorylation of 4E-BP1 by an mTOR-associated kinase.

FEBS letters ·Vol. 457 ·No. 3 ·1999-09-03 ·Pages 489-93

Heesom KJ, Denton RM

Abstract

mTOR immunoprecipitates contain two 4E-BP1 protein kinase activities. One appears to be due to mTOR itself and results in the phosphorylation of 4E-BP1 on residues T(36) and T(45), as shown previously by others. The other is a kinase which can be separated from mTOR and which phosphorylates 4E-BP1 within a peptide(s) containing residues S(64) and T(69). This phosphorylation, which occurs predominantly on S(64), results in the dissociation of 4E-BP1 from eIF-4E.

MeSH Terms
Amino Acid Sequence Animals Carrier Proteins Eukaryotic Initiation Factor-4E Intracellular Signaling Peptides and Proteins Male Molecular Sequence Data Peptide Initiation Factors/genetics,immunology,metabolism Phosphoproteins/genetics,immunology,metabolism Phosphorylation Phosphotransferases (Alcohol Group Acceptor)/isolation & purification,metabolism Precipitin Tests Protein Kinases/immunology,isolation & purification,metabolism Rats Rats, Wistar Recombinant Proteins/genetics,metabolism TOR Serine-Threonine Kinases
Chemicals
Carrier Proteins Eif4ebp1 protein, rat Eukaryotic Initiation Factor-4E Intracellular Signaling Peptides and Proteins Peptide Initiation Factors Phosphoproteins Recombinant Proteins Protein Kinases Phosphotransferases (Alcohol Group Acceptor) mTOR protein, rat TOR Serine-Threonine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heesom K J
Department of Biochemistry, School of Medical Sciences, University Walk, Bristol, UK. k.heesom@bris.ac.uk
Denton R M
Article Info
Journal
FEBS letters
Abbr.
FEBS Lett
ISSN
0014-5793
DOI
10.1016/s0014-5793(99)01094-7
Email
k.heesom@bris.ac.uk
Published
1999-09-03
Pages
489-93
Language
English
Region
England
NLM ID
0155157
Subset
IM
External Links
PubMed source DOI: 10.1016/s0014-5793(99)01094-7 View journal details
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