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PMID: 10464235 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A new class of cobalamin transport mutants (btuF) provides genetic evidence for a periplasmic binding protein in Salmonella typhimurium.

Journal of bacteriology ·Vol. 181 ·No. 17 ·1999-09-00 ·Pages 5539-41

Van Bibber M, Bradbeer C, Clark N, Roth JR

Abstract

No periplasmic binding protein has been demonstrated for the ATP-binding cassette (ABC)-type cobalamin transporter BtuCD. New mutations (btuF) are described that affect inner-membrane transport. The BtuF protein has a signal sequence and resembles the periplasmic binding proteins of several other ABC transporters.

MeSH Terms
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase ATP-Binding Cassette Transporters/genetics,metabolism Bacterial Proteins/genetics,metabolism Base Sequence Carrier Proteins/genetics,metabolism DNA, Bacterial Epistasis, Genetic Escherichia coli Proteins Gene Deletion Gene Expression Regulation, Bacterial Membrane Proteins/genetics,metabolism Methyltransferases Molecular Sequence Data Mutagenesis, Insertional Operon Periplasm/metabolism Salmonella typhimurium/genetics,metabolism Sequence Analysis, DNA Vitamin B 12/metabolism
Chemicals
ATP-Binding Cassette Transporters Bacterial Proteins BtuC peptide, E coli BtuD peptide, E coli BtuF protein, Salmonella typhimurium Carrier Proteins DNA, Bacterial Escherichia coli Proteins Membrane Proteins Methyltransferases 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase metE protein, E coli Vitamin B 12
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Van Bibber M
Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA.
Bradbeer C
Clark N
Roth J R
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.17.5539-5541.1999
Published
1999-09-00
Pages
5539-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94070
Subset
IM
Grants
NIGMS NIH HHS · R01 GM034804 · United States
NIGMS NIH HHS · GM34804 · United States
Databases
GENBANK
AF096877
External Links
PubMed source DOI: 10.1128/JB.181.17.5539-5541.1999 PMC Full Text View journal details
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