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PMID: 10460833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of human vascular endothelial cadherin glycans.

Glycobiology ·Vol. 9 ·No. 9 ·1999-09-00 ·Pages 915-25

Geyer H, Geyer R, Odenthal-Schnittler M, Schnittler HJ

Abstract

The glycosylation pattern of human vascular endothelial cadherin (VE-cadherin), purified from cultured human umbilical cord vein endothelial cells, was analyzed. VE-cadherin was metabolically radiolabeled with d-[6-(3)H]glucosamine, isolated by immunoprecipitation, purified by SDS-PAGE and in-gel digested with endoproteinase Asp N. Oligosaccharides were sequentially released from resulting glycopeptides and analyzed by chromatographic profiling. The results revealed that VE-cadherin carries predominantly sialylated diantennary and hybrid-type glycans in addition to some triantennary and high mannose-type species. Highly branched, tetraantennary oligosaccharides were found in trace amounts only. Immunohistochemical labeling of VE-cadherin and sialic acids displayed a codistribution along the intercellular junctions in endothelial cells of human umbilical arteries, veins, and cultured endothelial monolayers. Ca(2+)-depletion, performed on cultured endothelial cells, resulted in a reversible complete disappearance of VE-cadherin and of almost all sialic acid staining from the junctions. Sialidase treatment of whole cells caused a change of VE-cadherin immunofluorescence from a continuous and netlike superstructural organization to a scattered inconsistent one. Hence, cell surface sialic acids might play a role in VE-cadherin organization.

MeSH Terms
Actins/isolation & purification Antigens, CD Cadherins/chemistry Carbohydrate Sequence Cells, Cultured Endothelium, Vascular/chemistry Glycoproteins/chemistry Humans Intercellular Junctions/chemistry Molecular Sequence Data N-Acetylneuraminic Acid/isolation & purification Oligosaccharides/chemistry Umbilical Veins/cytology
Chemicals
Actins Antigens, CD Cadherins Glycoproteins Oligosaccharides cadherin 5 N-Acetylneuraminic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Geyer H
Institute of Biochemistry, Justus-Liebig-Universität Giessen, Friedrichstrasse 24, D-35392 Giessen, Germany.
Geyer R
Odenthal-Schnittler M
Schnittler H J
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Article Info
Journal
Glycobiology
Abbr.
Glycobiology
ISSN
0959-6658
DOI
10.1093/glycob/9.9.915
Published
1999-09-00
Pages
915-25
Language
English
Region
England
NLM ID
9104124
PMCID
PMC7108604
Subset
IM
External Links
PubMed source DOI: 10.1093/glycob/9.9.915 PMC Full Text View journal details
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