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PMID: 10460273 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Spinal substance P receptor expression and internalization in acute, short-term, and long-term inflammatory pain states.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 19 ·No. 17 ·1999-09-01 ·Pages 7670-8

Honor P, Menning PM, Rogers SD, Nichols ML, Basbaum AI, Besson JM, Mantyh PW

Abstract

Inflammatory pain involves the sensitization of both primary afferent and spinal cord neurons. To explore the neurochemical changes that contribute to inflammatory pain, we have examined the expression and ligand-induced internalization of the substance P receptor (SPR) in the spinal cord in acute, short-term, and long-term inflammatory pain states. These inflammatory models included unilateral injection of formalin (8-60 min), carrageenan (3 hr), and complete Freund's adjuvant (CFA; 3 d) into the rat hindpaw as well as adjuvant-induced polyarthritis (21 d). In acute inflammatory pain there is ongoing release of substance P (SP) as measured by SPR internalization in lamina I neurons at both 8 and 60 min after formalin injection. Although there is no tonic release of SP in short-term inflammatory pain, at 3 hr after carrageenan injection, SP is released in response to both noxious and non-noxious somatosensory stimulation with SPR internalization being observed in neurons located in both laminae I and III-IV. In long-term inflammatory pain models (CFA and polyarthritis) the same pattern of SP release and SPR activation occurs as is observed in short-term inflammation with the addition that there is a significant upregulation of the SPR in lamina I neurons. These results suggest that SPR internalization might serve as a marker of the contribution of ongoing primary afferent input in acute and persistent pain states. These stereotypical neurochemical changes suggest that there are unique neurochemical signatures for acute, short-term, and long-term inflammatory pain.

MeSH Terms
Acute Disease Afferent Pathways/physiology,physiopathology Animals Carrageenan Chronic Disease Formaldehyde Freund's Adjuvant Inflammation/physiopathology Male Neurons/physiology Pain/physiopathology Physical Stimulation Rats Rats, Sprague-Dawley Receptors, Neurokinin-1/genetics,metabolism Spinal Cord/physiology,physiopathology Time Factors
Chemicals
Receptors, Neurokinin-1 Formaldehyde Carrageenan Freund's Adjuvant
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Honor P
Neurosystems Center, Department of Preventive Sciences, Psychiatry and Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Menning P M
Rogers S D
Nichols M L
Basbaum A I
Besson J M
Mantyh P W
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Published
1999-09-01
Pages
7670-8
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6782496
Subset
IM
Grants
NIDA NIH HHS · DA11986 · United States
NIDCR NIH HHS · DE07288 · United States
DS NIH HHS · DS23970 · United States
External Links
PubMed source PMC Full Text View journal details
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