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PMID: 10456933 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Porin from Pseudomonas aeruginosa induces apoptosis in an epithelial cell line derived from rat seminal vesicles.

Infection and immunity ·Vol. 67 ·No. 9 ·1999-09-00 ·Pages 4794-800

Buommino E, Morelli F, Metafora S, Rossano F, Perfetto B, Baroni A, Tufano MA

Abstract

Micromolar concentrations of porin, purified from the outer membranes of Pseudomonas aeruginosa, induced in vitro the classic morphological and biochemical signs of apoptosis in an epithelial cell line (SVC1) derived from the rat seminal vesicle secretory epithelium. The programmed cell death (PCD) was p53 independent and associated with significant decrease of bcl-2 expression, a marked increase of c-myc transcriptional activity, and an absence of the mRNA coding for tissue transglutaminase. The Ca(2+) influx, caused by the porin treatment of SVC1 cells, appears to play an important role in the triggering of apoptosis in our biological model. The possibility that the porin property of inducing PCD plays a role in the infertility of individuals chronically infected by gram-negative bacteria is discussed.

MeSH Terms
Animals Apoptosis Calcium/metabolism Cell Line Cell Membrane/physiology Epithelial Cells/pathology Humans Male Porins/metabolism,pharmacology Proto-Oncogene Proteins c-myc/metabolism Pseudomonas aeruginosa Rats Seminal Vesicles/pathology Tumor Suppressor Protein p53/metabolism
Chemicals
Porins Proto-Oncogene Proteins c-myc Tumor Suppressor Protein p53 Calcium
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Buommino E
CNR International Institute of Genetics and Biophysics, Area di Ricerca del C.N.R., Medical School, 2nd University of Naples, Italy.
Morelli F
Metafora S
Rossano F
Perfetto B
Baroni A
Tufano M A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.67.9.4794-4800.1999
Published
1999-09-00
Pages
4794-800
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC96811
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.67.9.4794-4800.1999 PMC Full Text View journal details
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